Many forensic labs currently utilize PCR and capillary electrophoresis (CE)-based methods to analyze short tandem repeat (STR) markers and other polymorphisms. A major limitation of CE typing is that multiple polymorphisms cannot be analyzed together, causing forensic labs to validate and maintain multiple PCR-based systems. CE typing can often necessitate multiple rounds of testing.
Next-generation sequencing (NGS) enables single nucleotide polymorphism (SNP) and STR analysis for large numbers of globally relevant STR markers and dense SNP sets in a single test. The accuracy and high-throughput of Illumina sequencing by synthesis (SBS) chemistry promises to streamline STR and SNP forensic lab operations.
Illumina offers a complete, fully validated DNA-to-data solution designed for forensic genomics. First, forensically relevant STRs and SNPs are simultaneously PCR amplified, with indexed adapters incorporated into each amplicon in a separate round of PCR.
Subsequent DNA cluster generation and sequencing by synthesis is performed on the MiSeq FGx Instrument. Finally, analyze and interpret your sequencing data off-line using ForenSeq Universal Analysis Software.
Click on the below to view products for each workflow step.
We recommend using your current DNA extraction and quantitation methods.
Assay approximately 200 genetic markers in a single test. Includes all reagents to prepare sequencing libraries from forensic DNA samples.
Targeted sequencing of forensically relevant STR and SNP loci empowers you to generate leads, even on cold cases. Learn More »
NGS can help labs produce high-quality forensic profiles for national DNA databases, with a faster turnaround. Learn More »
NGS delivers a single platform and workflow to overcome the wide range of difficult samples encountered in missing persons cases. Learn More »
Genetic analysis of mass fatality evidence with NGS lets you extract as much information as possible from highly compromised samples.
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