The HiSeq 3000/HiSeq 4000 Sequencing Systems build on the proven performance of the HiSeq 2500 System and leverage the patterned flow cell technology of the ultra-high-throughput HiSeq X Systems to deliver high sequencing speed and performance. Offering high coverage, fast turnaround times, and the flexibility to process various sample types, the HiSeq 3000/HiSeq 4000 Systems provide a multi-application solution for production-scale genomic laboratories.
The HiSeq 3000/HiSeq 4000 Systems deliver high-throughput at a low price per sample across a range of genomic applications. The HiSeq 4000 System can sequence up to 12 genomes, 100 transcriptomes, or 96 exomes in fewer than 3.5 days. This high capacity means more samples can be sequenced simultaneously at a greater depth, generating rich and meaningful data.
The dual-flow cell HiSeq 4000 System can process one or two flow cells in parallel and provides high output and daily throughput with a capacity of 1.5 Tb and 5 billion reads per run. The HiSeq 3000 System offers the same output per flow cell as the HiSeq 4000 System, but only processes one flow cell at a time, delivering 750 Gb and 2.5 billion reads per run. When throughput needs increase, the HiSeq 3000 System can be upgraded to a HiSeq 4000 System.
Illumina offers a full range of HiSeq 3000/HiSeq 4000 software solutions to help you go from biological samples to meaningful results. BaseSpace Sequence Hub provides an integrated solution for the analysis and storage of Illumina sequencing data. With whole-genome sequencing,exome/enrichment, and RNA-Seq apps, BaseSpace Sequence Hub streamlines NGS data analysis while providing an efficient means for data storage, sharing, and transfer.
Learn how DNA sequencing is leading to progress in the rapid identification of variants that might be involved in genetic diseases. Thanks to this work, Dr. Kingsmore and his team now hold a Guinness World Records title.View Video
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A Garvan Institute sarcoma study identifies rare cancer-associated variants in the germline with the HiSeq 2500 System.Read Article
Includes sequencing reagents specifically designed for the HiSeq 3000/HiSeq 4000 Systems. Enables production-scale sequencing for diverse applications.
This kit enables cluster generation for subsequent sequencing on the HiSeq 3000 and HiSeq 4000 Systems.
Provides reagents for Single Read (SR) cluster generation on the cBot2 System. Cluster reagents are compatible with the HiSeq 3000 and HiSeq 4000 Systems.