AmpliSeq for Illumina DNA Library Prep Training
|AmpliSeq for Illumina Custom DNA Panel||AmpliSeq for Illumina On-Demand||Illumina DNA Prep with Enrichment||Nextera Rapid Capture Custom Enrichment Kit|
|Content Specifications||Custom content of interest - up to 5 Mb||Custom content from 1 (24 amplicons) to 500 (15,000 amplicons) genes||Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Custom: 0.5 - 15 Mb genomic content of interest.
Fixed panels: Content varies by panel.
|Custom genomic content of interest (0.5 - 15 Mb)|
|Description||A custom research assay that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy.||A custom research assay from >5000 pretested genes that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy.||A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing.||A custom research workflow that utilizes custom panels, existing Illumina designed panels or previously ordered custom projects to enable enrichment of targeted genomic content.|
|Input Quantity||1–100 ng (10 ng recommended per pool)||1-100 ng DNA||10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide).||50 ng DNA|
|Mechanism of Action||Multiplex PCR||Multiplex PCR||Bead-bound transposomes and hybrid-capture chemistry||Enzymatic fragmentation and hybridization-based enrichment|
|Method||Amplicon Sequencing , Custom Sequencing, Genotyping by Sequencing , Targeted DNA Sequencing||Amplicon Sequencing , Targeted DNA Sequencing||Custom Sequencing, Exome Sequencing , Target Enrichment , Target Enrichment, Targeted DNA Sequencing||Custom Sequencing, Target Enrichment , Target Enrichment, Targeted DNA Sequencing|
|Multiplexing||Up to 96-plex||Up to 96-plex||Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested.||12-plex (pool up to 12 samples per enrichment reaction)|
|Number of Reactions||750 or 3000 reactions||24 or 96 reactions||Custom workflow: 16 or 96 samples||48, 96, or 288 samples|
|Specialized Sample Types||Blood, FFPE Tissue||Blood, FFPE Tissue||Blood, FFPE Tissue, Saliva||Not FFPE-Compatible|
|Species Category||Any Species, Bovine, Canine, Chicken, Hamster, Human, Maize, Mouse, Ovine, Porcine, Rice, Soybean, Tomato||Human||Human, Other||Human|