AMEA Illumina Solutions Center

Overview of complete DNA NGS workflows (sample prep, sequencers, and data analysis) with a focus on experimental design and everything that you should consider to be successful in implementing NGS in your own lab.

This Illumina Solutions Center training is a mix of wet lab work & theory, taught by a mix of product experts, covering the range of sequencing & DNA library preparation solutions and recommended 3rd party equipment.

Who is this training for?

Life scientists who have no or limited experience with NGS, but who would like to gain an understanding of the technology.

What will you learn?

A practical understanding of lllumina's DNA sequencing workflows.

Format & contents

This training is delivered in-person at the Illumina Solution Center over 2-days, covering:

• Theory of Illumina NGS & sequencing workflow
• Overview of DNA library preparation methods
• Lab based: Full run set up, QC & monitoring
• NGS Experimental design
• Cloud and local analysis options
• Customer Support Resources

This course is highly interactive and class size is limited to 6 students. Exercises and knowledge checks are built into each session.

Overview of the complete RNA NGS workflow with a focus on experimental design and everything you should consider to be successful in NGS. The class covers possible applications of RNA NGS, experimental design considerations, Illumina’s sequencing workflow (incl. library prep and instrumentation), and data analysis workflow.

This Illumina Solutions Center training is a mix of wet lab work & theory, taught by a mix of product experts, covering the range of sequencing & library preparation solutions and recommended 3rd party equipment.

Who is this training for?

Life scientists who have no or limited experience with NGS, but who would like to gain an understanding of the technology.

What will you learn?

A practical understanding of lllumina's RNA sequencing workflows.

Format & contents

This training is delivered in-person at the Illumina Solution Center over 2-days, covering:

• Theory of Illumina NGS & sequencing workflow
• Overview of RNA library preparation methods
• Lab based: Full run set up, QC & monitoring
• NGS Experimental design
• Cloud and local analysis options
• Customer Support Resources

This course is highly interactive and class size is limited to 10 students. Exercises and knowledge checks are built into each session.

An overview of a comprehensive sequencing workflow including a comparison of traditional methods used in microbiology labs vs. NGS approach; outline of different NGS strategies, sample preparation methods and sequencing instruments; experimental design considerations for microbiology samples, and introduction to NGS data analysis.

This Illumina Solutions Center training is a mix of wet lab work & theory, taught by a mix of product experts, covering the range of sequencing & library preparation solutions and recommended 3rd party equipment.

Who is this training for?

Life scientists who have no or limited experience with NGS, but who would like to gain an understanding of the technology.

What will you learn?

A practical understanding of lllumina's sequencing workflows applicable to microbiology.

Format & contents

This training is delivered in-person at the Illumina Solution Center over 2-days, covering:

• Theory of Illumina NGS & sequencing workflow
• NGS application in microbiology
• Lab based: Full run set up, QC & monitoring
• Overview of library preparation methods
• NGS Experimental design
• Cloud and local analysis options

This course is highly interactive and class size is limited to 10 students. Exercises and knowledge checks are built into each session.

In this training you will find out how to evaluate quality of sequencing data, what data format is needed to start secondary analysis and how to manipulate FASTQ files in order to improve alignment. Participants will learn what happens during secondary analysis, what metrics are generated during this process, sample and lab processes, and how to identify important variants. The training is introductory but basic knowledge of the sequencing workflow would be recommended.

This Illumina Solutions Center training is a mix of wet lab work & theory, taught by a mix of product experts, covering the range of sequencing & library preparation solutions and recommended 3rd party equipment.

Who is this training for?

Researchers who would like to start analyzing sequencing data or to better understand analysis to improve communication with internal bioinformatics departments.

What will you learn?

A practical understanding of lllumina's data analysis offerings.

Format & contents

This training is delivered in-person at the Illumina Solution Center over 1.5 days, covering:

• Basics of data analysis workflow
• How to evaluate sequencing data with SAV software
• Practical tips for choosing a solution for data analysis
• How to generate, evaluate and manipulate FASTQ files
• Secondary analysis metrics and QC
• What happens during tertiary data analysis

This course is highly interactive and class size is limited to 10 students. Exercises and knowledge checks are built into each session.

Use with genomic or FFPE DNA for whole-exome, custom, and fixed panel targeted sequencing.

Illumina DNA Prep with Enrichment is the fastest and most flexible targeted sequencing solution for DNA in the Illumina library prep portfolio. It combines optimized performance using bead-based library prep chemistry for reliable & fast results, 85% quicker than previous Illumina enrichment, and integrated DNA extraction protocols for blood & saliva.

This Illumina Solutions Center training is a mix of wet lab & theory, taught by a mix of product experts, running control samples through the complete workflow using all recommended 3rd party equipment.

Who is this training for?

1. Lab technicians about to use the Illumina DNA Prep with Enrichment kit.
2. Users who want to understand every aspect of the protocol.

What will you learn?

Theory, best practice and analysis options for this workflow.

Format & contents

This training is delivered in-person at the Illumina Solution Center over 1.5 days, covering:

• Library preparation theory & best practices
• Lab based: Input DNA QC
• Lab based: DNA tagmentation, cleanup & QC
• Lab based: Hybridization & Enrichment
• Lab based: Final QC and ready for sequencing
• Sequencing & Analysis workflow

Additional content can be customized based on individual customer needs.

Use with DNA from low-input, blood or saliva samples for whole-genome sequencing.

A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing.

• Fast, automatable workflow, with ~1.5 hours total time.
• Flexibility to accommodate variations in sample type, DNA input amount (25ng to 300ng), and application.
• Optimized library prep performance, generating reliable results.

This Illumina Solutions Center training is a mix of wet lab & theory, taught by a mix of product experts, running control samples through the complete workflow using all recommended 3rd party equipment.

Who is this training for?

1. Lab technicians about to use the Illumina DNA Prep with Enrichment kit.
2. Users who want to understand every aspect of the protocol.

What will you learn?

Theory, best practice and analysis options for this workflow.

Format & contents

This training is delivered in-person at the Illumina Solution Center over 1.5 days, covering:

• Library preparation theory & best practices
• Lab based: Input DNA QC
• Lab based: Full run set up, QC & monitoring
• Lab based: Library pooling
• Lab based: Final QC and ready for sequencing

Additional content can be customized based on individual customer needs, e.g. Multiplexing strategies; Sequencing of low diversity libraries; Library validation.

The iSeq 100 Sequencing System makes next-generation sequencing (NGS) easier and more affordable than ever. Designed for simplicity, it allows labs to sequence DNA and RNA including a variety of applications.

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This Illumina Solutions Center training is a mix of wet lab work & theory, taught by product experts, using a qualified instrument and all recommended 3rd party equipment.

Who is this training for?

1. New owners of iSeq 100 system
2. New staff in lab with existing system

What will you learn?

Best practices and operation of complete iSeq 100 workflow.

Format & contents

This training is delivered in-person at the Illumina Solution Center over 1.5 days, covering:

• Sequencing technology
• Best practices for iSeq 100 operation & maintenance
• Lab based: Full run set up, QC & monitoring
• Cloud & local analysis options
• Library preparation
• Customer Support Resources

Additional content can be customized based on individual customer needs, e.g. Multiplexing strategies; Sequencing of low diversity libraries; Library validation.

The MiniSeq System delivers the quality and reliability of Illumina next-generation sequencing (NGS) technology in a powerful, accessible benchtop sequencer with a small footprint.

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This Illumina Solutions Center training is a mix of wet lab work and theory, taught by product experts, using a qualified instrument and all recommended 3rd party equipment.

Who is this training for?

1. New owners of MiniSeq system (incl. in purchase)
2. New staff in lab with existing system

What will you learn?

Best practices and operation of complete MiniSeq workflow.

Format & contents

This training is delivered in-person at the Illumina Solution Center. 1.5 days, covering:

• Illumina Sequencing technology
• Best practices for MiniSeq operation & maintenance
• Lab based: Full run set up, QC and monitoring
• Cloud and local analysis options
• Customer Support Resources

Additional content can be customized based on individual customer needs, e.g. Multiplexing strategies; Sequencing of low diversity libraries; Library validation.

The MiSeq System facilitates your research with a wide range of sequencing applications. This benchtop sequencer produces up to 15 Gb per run with read lengths of up to 2x300bp.

View product page

This Illumina Solutions Center training is a mix of wet lab work and theory, taught by product experts, using a qualified instrument and all recommended 3rd party equipment.

Who is this training for?

1. New owners of MiSeq system (incl. in purchase)
2. New staff in lab with existing system

What will you learn?

Best practices and operation of complete MiSeq workflow.

Format & contents

In person at a Solutions Center. 1.5 days, covering:

• Illumina Sequencing technology
• Best practices for MiSeq operation & maintenance
• Lab based: Full run set up, QC & monitoring
• Cloud and local analysis options
• Library preparation overview
• Customer Support Resources

Additional content can be customized based on individual customer needs, e.g. Multiplexing strategies; Sequencing of low diversity libraries; Library validation.

The MiSeqDx instrument is the first Food and Drug Administration (FDA)-regulated and Conformite Europeene in vitro diagnostic (CE-IVD)-marked platform for NGS. Since then, it has also been registered in many countries across the globe. Designed specifically for clinical laboratories, the MiSeqDx System delivers a broad range of sequencing applications.

View product page

This Illumina Solutions Center training is a mix of wet lab work and theory, taught by product experts, using a qualified instrument and all recommended 3rd party equipment.

Who is this training for?

1. New owners of MiSeqDx system (incl. in purchase)
2. New staff in lab with existing system

What will you learn?

Best practices and operation of complete MiSeqDx workflow.

Format & contents

In person at a Solutions Center. 1.5 days, covering:

• Illumina Sequencing technology
• Best practices for MiSeqDx operation & maintenance
• Lab based: Full run set up, QC & monitoring
• Sequencing Theory
• Cloud and local analysis options
• Customer Support Resources

Additional content can be customized based on individual customer needs, e.g. Multiplexing strategies; Sequencing of low diversity libraries; Library validation.

The NextSeq 550 System combines tried-and-true instrument technologies and tunable output with sequencing and array capabilities. Users can run transcriptome, targeted resequencing, genotyping, and other applications all on one platform.

View product page

This Illumina Solutions Center training is a mix of wet lab work and theory, taught by product experts, using a qualified instrument and all recommended 3rd party equipment.

Who is this training for?

1. New owners of NextSeq 550 system (incl. in purchase)
2. New staff in lab with existing system

What will you learn?

Best practices and operation of complete NextSeq 550 sequencing workflow (no arrays).

Format & contents

In person at a Solutions Center. 1.5 days, covering:

• Illumina Sequencing technology
• Best practices for NextSeq 550 operation & maintenance
• Lab based: Full run set up, QC & monitoring
• Cloud and local analysis options
• Library preparation overview
• Customer Support Resources

Additional content can be customized based on individual customer needs, e.g. Multiplexing strategies; Sequencing of low diversity libraries; Library validation.