Products

Pillar® oncoReveal™ Multi-Cancer CNV + RNA Fusion Panel

A pan-cancer panel with combined DNA/RNA content, utilizing the single-tube SLIMamp™ workflow for fast library prep and turnaround time.

~6.5 hr

Assay time

~3 hr

Hands-on time

5-80ng DNA, 10-50ng RNA

Input quantity

See full details in the specifications table

Overview

The oncoReveal™ Multi-Cancer with CNV and RNA Fusion Panel is a combined DNA/RNA multi-cancer panel. The assay combines the DNA-based ONCO/Reveal Multi-Cancer v4 with CNV Panel with the ONCO/Reveal Multi-Cancer RNA Fusion v2 Panel allowing for joint sequencing. The assay uses proprietary Stem Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.

  • Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS lab

  • Achieve variant detection as low as 1% VAF without UIDs, even with limited DNA input or poor sample quality

  • Improve lab efficiency and reduce “no calls”, repeat testing and difficult interpretation decisions

  • < 8 hours DNA to sequencer, < 9-10 hours RNA to sequencer

Specifications

Assay time ~6.5 hr
Cancer type Pan-Cancer, Solid Tumor
Content specifications 78- gene small pan-tumor oncology panel
Description 78- gene amplicon-based panel with DNA & RNA content, simple single tube workflow
Hands-on time ~3 hr
Input quantity 5-80ng DNA, 10-50ng RNA
Instruments MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode
Mechanism of action Multiplex PCR using tiled amplicon
Method Targeted RNA Sequencing, Amplicon Sequencing, Targeted DNA Sequencing
Nucleic acid type DNA, RNA
Number of amplicons Pool 1 (DNA): 341 amplicons. Pool 2 (RNA): <80 amplicons plus MET exon 14 skipping
Number of reactions 24 DNA/24 RNA
On-target reads 99.5% ± 0.1%
Specialized sample types Blood, Low-Input Samples, FFPE Tissue
Species category Human
Target insert size Average: 120bp (range 86-185bp)
Technology Sequencing
Uniformity 98.2% ± 0.7%
Variant class Gene fusions, Somatic variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs)

Required products

Pillar® oncoReveal™ panels require the use of a Pillar index kit.

 

What accessories do I need?

Pillar® oncoReveal™ panels require the use of a Pillar index kit. Pick your preferred number of reactions.

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Applications

Example workflow

1
Prep

Pillar® oncoReveal™ Multi-Cancer CNV + RNA Fusion Panel

2
Sequence
3
Analyze

Documentation

Product literature

Literature is not currently available for this product.

Support documentation

Figures

Gene list

Panel specifications

Pillar® oncoReveal™ Multi-Cancer CNV + RNA Fusion Panel (24 reactions)

HNA-HS-1001-48

Pillar® oncoReveal™ Multi-Cancer CNV + RNA Fusion Panel (24 reactions)

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Indices PI501-8, PI701-4 (32 combinations, 96 reactions)

IDX-PI-1001-96

Indices PI501-8, PI701-4 (32 combinations, 96 reactions)

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Kit D, indices PI501-8, PI701-12

IDX-PI-1004-192

Kit D, indices PI501-8, PI701-12

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