Products

Pillar® oncoReveal™ Myeloid Panel

A 58-gene pan-cancer panel with combined DNA content, utilizing the single-tube SLIMamp™ workflow for fast library prep and turnaround time.

~6.5 hr

Assay time

~3 hr

Hands-on time

10ng - 80ng DNA (20ng recommended)

Input quantity

See full details in the specifications table

Overview

The oncoReveal™ Myeloid Panel is a DNA NGS sequencing panel using proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.

  • Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS lab

  • Achieve variant detection as low as 1% VAF without UIDs, even with limited DNA input or poor sample quality

  • Improve lab efficiency and reduce “no calls”, repeat testing and difficult interpretation decisions

  • < 8 hours DNA to sequencer

Specifications

Assay time ~6.5 hr
Cancer type Pan-Cancer, Solid Tumor
Description 58-gene amplicon based DNA panel, simple single tube workflow
Hands-on time ~3 hr
Input quantity 10ng - 80ng DNA (20ng recommended)
Instruments MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode
Mechanism of action Multiplex PCR using tiled amplicon
Method Amplicon Sequencing, Targeted DNA Sequencing
Nucleic acid type DNA
Number of amplicons 1 pool, 766 amplicons
Number of reactions 24 reactions
On-target reads 99.6% ± 0.2%
Specialized sample types Blood, Low-Input Samples
Species category Human
Target insert size Average: 215 bp
Technology Sequencing
Uniformity 96.8% ±1.0%
Variant class Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs)

Required Products

Pillar® oncoReveal™ panels require the use of a Pillar index kit. Pick your preferred number of reactions.

of

Applications

Example workflow

1
Prep

Pillar® oncoReveal™ Myeloid Panel

2
Sequence
3
Analyze

Documentation

Product literature

Literature is not currently available for this product.

Support documentation

Compare

Pillar® oncoReveal™ Myeloid Panel AmpliSeq for Illumina Myeloid Panel TruSight Myeloid Sequencing Panel
Assay time ~6.5 hr 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 8 hours
Cancer type Pan-Cancer, Solid Tumor Hematologic Hematologic
Description 58-gene amplicon based DNA panel, simple single tube workflow Targeted panel to study 40 DNA genes, 29 RNA fusion driver genes, and 5 gene expression levels associated with myeloid cancers. For somatic mutation detection studies in myeloid malignancies.
Hands-on time ~3 hr < 1.5 hr 3 hr
Input quantity 10ng - 80ng DNA (20ng recommended) 20 ng high-quality DNA; 10 ng high-quality RNA
(10 ng recommended per pool)		 50 ng
Instruments MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode MiSeq System, MiniSeq System MiSeq System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System
Mechanism of action Multiplex PCR using tiled amplicon
Method Amplicon Sequencing, Targeted DNA Sequencing Targeted RNA Sequencing, Amplicon Sequencing, Targeted DNA Sequencing Amplicon Sequencing, Targeted DNA Sequencing
Nucleic acid type DNA DNA, RNA DNA
Number of amplicons 1 pool, 766 amplicons
Number of reactions 24 reactions
On-target reads 99.6% ± 0.2%
Specialized sample types Blood, Low-Input Samples Blood, Not FFPE-Compatible, Bone Marrow Not FFPE-Compatible
Species category Human Human Human
Target insert size Average: 215 bp
Technology Sequencing Sequencing Sequencing
Uniformity 96.8% ±1.0%
Variant class Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels) Somatic variants, Insertions-deletions (indels)

Figures

Gene list

Panel specifications

oncoReveal™ Myeloid Panel

HDA-MY-1001-24

oncoReveal™ Myeloid Panel

Sign in to add to cart or see pricing.

Request pricing

List Price:

Discounts:

Indices PI501-8, PI701-4 (32 combinations, 96 reactions)

IDX-PI-1001-96

Indices PI501-8, PI701-4 (32 combinations, 96 reactions)

Sign in to add to cart or see pricing.

Request pricing

List Price:

Discounts:

Kit D, indices PI501-8, PI701-12

IDX-PI-1004-192

Kit D, indices PI501-8, PI701-12

Sign in to add to cart or see pricing.

Request pricing

List Price:

Discounts:

Showing of

Selection summary

Product

Qty

Unit Price

Total ( items)

You have not selected any items yet.

Please add these products to your cart before leaving the page to avoid losing them.

Frequently purchased

Order the NovaSeq X Series

Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.

MiSeq Reagent Kit v3

Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.

TruSight Oncology 500

Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.

Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

Speak with a specialist

Reach out for information about our products and services, or get answers to questions about our technology.