Biomarker Discovery Tools for Immuno-Oncology Research
Tumor tissues vary considerably in their microenvironment, and the existence of other cell types can influence the ability of the immune system to infiltrate and attack tumor cells. As promising new therapies evolve, there is an increasing need to identify cancer immunotherapy biomarkers to guide their appropriate application.
NGS can provide careful analysis of the cancer genome, and efficiently assess the tumor milieu as a real-time, highly sensitive monitor of immune marker expression in response to tumor growth or treatment. NGS analysis can be used to characterize the immune cell repertoire, identify various cell populations in the microenvironment, and comprehensively quantify gene expression of thousands of targets simultaneously.
Featured Publications: Cancer Immunotherapy Research
High-throughput sequencing has shown remarkable utility in cancer and immunology research, as well as in the development of individualized immunotherapy.
Epigenetic Analysis of Cancer Samples
Studies of epigenetic alterations in cancer, such as aberrant methylation and altered transcription factor binding, can provide insight into important tumorigenic pathways. As altered methylation often activates or silences genes, changes in the epigenome can affect gene expression and the rate of cancer progression.
NGS and microarray technologies can detect altered methylation patterns and other epigenetic changes in cancer.
Surveying the Chromatin Landscape with NGS
Researchers at Stanford University develop novel sequencing methods to understand the epigenome and its impact on cancer and immune disease.
RNA Sequencing
RNA analysis may help to identify aspects of the tumor microenvironment that can influence the effectiveness of checkpoint immunotherapies, such as inductive and inhibitory cytokines, and local recruitment of other cells types that can inhibit the T-cell response.
Keeping Pace with Immuno-oncology Research Breakthroughs
NGS technology can accelerate biomarker identification and bring down costs for research subject screening and safety monitoring.
Translational Research
Whether you work to develop new therapies, study the progression of cancer, or simply want to better understand the mechanisms of disease, Illumina translational research informatics platforms transform complex genomic data into meaningful insights.
Informatics Applications for Immuno-oncology Research Using NGS
Improved informatics tools enable neoantigen discovery and tumor microenvironment analysis.
Tumor Microenvironment Analysis Workflow
Illumina offers several library preparation and sequencing options with access to data analysis options for tumor microenvironment analysis. Streamlined workflows and flexible kit configurations accommodate multiple study designs.
Approximately 90% of the world’s sequencing data are generated using Illumina sequencing by synthesis (SBS) chemistry.*
Click on the below to view products for each workflow step.
TruSeq Exome
TruSeq Exome Library Prep Kit is a cost-effective library preparation and exome enrichment solution.
RNA AccessThe TruSeq RNA Access library prep kit provides a low-cost solution for analyzing human RNA isolated from limited or low-quality samples, including FFPE.
Illumina Bio-Rad® SureCell™ WTA 3′ Library Prep Kit for the ddSEQ™ System
Transcriptome profiling of hundreds to tens of thousands of single cells in a single experiment.
TruSeq DNA Methylation KitWhole-genome bisulfite sequencing libraries from as little as 50 ng DNA.
NextSeq Series
Flexible configurations that support up to 12 exomes per run.
HiSeq 2500 SystemHigh throughput on a scalable platform for sequencing dozens to hundreds of cancer exomes per run.
HiSeq 3000/HiSeq 4000 Systems
Patterned flow cell technology results in high throughput, low price per sample, and enhanced speed and performance.
NovaSeq SeriesFlexibility and unprecedented throughput for virtually any genome, sequencing method, and scale of project.
BaseSpace RNA-Seq Apps
Immuno-Oncology filter for RNA sequencing analysis.
Isaac Enrichment App
Uses the Isaac Genome Alignment Software and the Isaac Variant Caller for exome data analysis.
Featured Publications
Cancer immunotherapy. A dendritic cell vaccine increases the breadth and diversity of melanoma neoantigen-specific T cells.
Science 348 803-8 2015
Comprehensive analysis of cancer-associated somatic mutations in class I HLA genes.
Nat Biotechnol 33 1152-8 2015
The epigenetic landscape of T cell exhaustion.
Science 354 1165-1169 2016
Additional Resources
Genomic Technologies for Cancer Research
Learn the numerous ways that genomic technologies can be applied to tumor biology.
Immuno-oncology Research Informatics Applications Using NGS
Improved informatics tools enable neoantigen discovery and tumor microenvironment analysis
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Sign Up*Data calculations on file, Illumina, Inc, 2015.