Scalable throughput for studies of all sizes
So many ways to sequence. Scale up and down effortlessly with our improved economics and an output of up to 6 Tb and 20 B reads in < 2 days.
Broad, deep sequencing for bigger discoveries
Go where discoveries are made. Accelerate your science with immense sequencing breadth and depth when you need it. All on one instrument.
Flexible performance for mix and match options
Do what you want. Mix and match flow cell types, run one or two flow cells at a time, choose between multiple read lengths, workflows, and more.
Streamlined operations for increased efficiency
You're in control. Improved sequencing costs via our new reagent kits. Automated flow cell loading. Open platform for build-your-own applications. We're dedicated to helping you maximize efficiency at every turn.
Advanced applications are the key to groundbreaking discoveries
NovaSeq 6000 offers deeper and broader coverage through advanced applications for a comprehensive view of the genome. Unlock the full spectrum of genetic variation and biological function with high-throughput sequencing.
See All ApplicationsNovaSeq performs whole-genome sequencing more efficiently and cost-effectively than ever. Its scalable output generates up to 6 Tb and 20 billion reads in dual flow cell mode with simple streamlined automated workflows. Configure the system to sequence a trio in one day or up to 48 genomes in ~2 days for the most comprehensive coverage.
Prep
Illumina DNA PCR-Free Prep
A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing or tumor-normal sequencing.
llumina DNA Prep
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
Sequence
NovaSeq Reagent Kits v1.5
New kits feature updated reagent formulations with 2x longer shelf life and improved sequencing costs to help power your most impactful studies.
Analyze
*Time per sample, assuming 30x coverage/sample
DRAGEN Germline Pipeline (on BSSH or on DRAGEN server)
The DRAGEN Germline Pipeline v3 aligns and optionally variant calls FASTQ files, outputting a BAM, VCF, or both.
DRAGEN Somatic Pipeline (on BSSH or on DRAGEN server)
The DRAGEN Somatic Pipeline aligns and variant calls Tumor-only or Tumor/Normal FASTQ files, outputting BAM(s) and a VCF.
Representing less than 2% of the genome, WES is a cost-effective alternative to WGS. The NovaSeq System offers sequencing of up to 500 exomes in a single run using a dual S4 flow cell.
Prep
*2.5 days total prep time, 6 hours hands-on time
Illumina DNA Prep with Enrichment
This kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry is combined with a simplified, single hybridization protocol to reduce total workflow time.
Sequence
NovaSeq Reagent Kits v1.5
New kits feature updated reagent formulations with 2x longer shelf life and improved sequencing costs to help power your most impactful studies.
Analyze
*Time per sample, assuming 100x coverage/sample
DRAGEN Enrichment
This app aligns and optionally variant calls FASTQ, BAM or CRAM files, outputting a BAM, VCF, or both.
Detect coding and multiple forms of noncoding RNA in normal or low-quality samples. With the NovaSeq System, you can sequence up to 400 transcriptomes in a single run using a dual S4 flow cell.
Prep
Illumina Stranded Total RNA Prep
Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with unparalleled study flexibility.
Sequence
NovaSeq Reagent Kits v1.5
New kits feature updated reagent formulations with 2x longer shelf life and improved sequencing costs to help power your most impactful studies.
Analyze
*Time per sample, assuming 200x coverage/sample
DRAGEN RNA Pipeline
Performs alignment, quantification, and fusion detection.
Innovation-driven performance
System specifications range
Output range
80 - 6000 Gb
Paired end reads per run
1.6 - 40B
Max read length
2 × 250 bp
Run time
13 - 44 hours
A streamlined, flexible workflow designed to help you accelerate your research and maximize results
Manage Workflow
Manage your workflow seamlessly with BaseSpace Clarity LIMS or your existing LIMS system.
Prepare Libraries
Prepare libraries with a broad range of high-performance library preparation kits.
Sequence
Generate sequences with ready-to-use reagents featuring proven Illumina sequencing by synthesis chemistry.
Analyze Data
Leverage the Illumina DRAGEN Bio-IT Platform or BaseSpace Sequence Hub for accurate, rapid secondary analysis.
Interpret Results
Interpret results, get insights, and drive discovery with BaseSpace Variant Interpreter, BaseSpace Cohort Analyzer, and TruSight Software Suite.
Support for your science
Products & Services
NovaSeq 6000 v1.5 Reagent Kits
New kits feature updated reagent formulations with 2x longer shelf life and improved sequencing costs to help power your most impactful studies.
NovaSeq Xp Workflow
The NovaSeq Xp workflow enables labs to load libraries directly into each lane of a NovaSeq 6000 System flow cell, without additional instrumentation.
Illumina RNA Prep with Enrichment
Achieve rapid, targeted interrogation of an expansive number of target genes with exceptional capture efficiency and coverage uniformity.
Illumina DNA PCR-Free Prep
A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing.
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Customer Stories
Go behind the science
See what's possible with the power of NovaSeq 6000 and NGS technology
See All StoriesRady Children’s Institute for Genomic Medicine is using the DNA PCR-Free Prep kit for NovaSeq 6000 to make a potentially record-breaking time-to-diagnosis for children with rare genetic disorders
Read MoreCole Trapnell, PhD is transforming the world of biology by making bioinformatics tools available to all
Read MoreOmniSeq, Inc. scaled up to process a large number of both tissue and blood plasma samples every week, batching from 16 to 192 samples in a single run on NovaSeq 6000 with the TruSight Oncology 500 suite of products
Read MoreGenomics England’s human genome initiative (GenOMICC) is launching a new study aimed at uncovering genetic factors in susceptibility to COVID-19, furthering our understanding of the pandemic through genomics
Read MoreThe DRAGEN platform enables GeneDx to scale to whole-genome analysis and identify variants with precision
Read More
Be a part of the Illumina online community
Join a community of like-minded customers using Illumina sequencers, library preparation kits, and automated data analysis to fuel groundbreaking research. Discuss best practices, troubleshoot, and collaborate with Illumina scientists, developers, and each other.
Learn MoreFeatured Products
NovaSeq Reagent Kits
Reagent kits for the NovaSeq 6000 System provide ready-to-use cartridge-based reagents for cluster generation and SBS.
View ProductsNovaSeq Xp Workflow
The NovaSeq Xp workflow enables labs to load libraries directly into each lane of a NovaSeq 6000 System flow cell, without additional instrumentation.
View Product
TruSeq Stranded Total RNA
A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
View Product
Nextera XT DNA Library Preparation Kit
Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in as little as 90 minutes, with a low DNA input requirement
View Product