The AmpliSeq for Illumina Comprehensive Cancer Panel provides a targeted resequencing solution for analyzing somatic mutations across 409 genes with known associations to cancer. Key features include:
The Comprehensive Cancer Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This panel detects variants within multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate malignancies. The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
| 装置 | 推奨サンプル数 | リード長 |
|---|---|---|
| NextSeq 550 System | Samples per run: mid output: 4, high output: 12 (assumes minimum coverage of 500×) | 2 × 150 bp |
| AmpliSeq for Illumina Comprehensive Cancer Panel | AmpliSeq for Illumina Cancer Hotspot Panel v2 | |
|---|---|---|
| Assay Time | 6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) |
| Cancer Type | Pan-Cancer, Solid Tumor | Pan-Cancer, Solid Tumor |
| Content Specifications | Full exon coverage of 409 genes associated with cancer. | Hotspot regions of 50 genes with known associations to cancer |
| Description | Somatic analysis research across pan-cancer genes. | Somatic analysis research into hotspot regions of 50 cancer-related genes. |
| Hands-On Time | < 1.5 hours | < 1.5 hours |
| Input Quantity | 1–100 ng (10 ng recommended per pool) | 1–100 ng (10 ng recommended per pool) |
| Method | Amplicon Sequencing , Targeted DNA Sequencing | Amplicon Sequencing , Targeted DNA Sequencing |
| Multiplexing | 96 dual index combinations | 96 dual index combinations |
| Specialized Sample Types | Blood, FFPE Tissue | FFPE Tissue |
| Species Category | Human | Human |
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
Sample Identification with the AmpliSeq for Illumina Sample ID Panel Application Note
Application Note | HTML
AmpliSeq for Illumina Comprehensive Cancer Panel Data Sheet
Data Sheet | HTML
AmpliSeq for Illumina Comprehensive Cancer Panel Gene List
product_file | EXCEL< 1 MB
NextSeq 550 amplicon sequencing solution
Application Note | PDF< 1 MB
AmpliSeq for Illumina Comprehensive Cancer Panel Reference Guide Documentation
AmpliSeq for Illumina Direct FFPE DNA Kit Reference Guide Documentation
AmpliSeq for Illumina Comprehensive Cancer Panel Consumables & Equipment Documentation
AmpliSeq for Illumina Comprehensive Cancer Panel Checklist Documentation
Illumina Adapter Sequences Documentation
AmpliSeq for Illumina Comprehensive Cancer Panel Support - Documentation & Literature
Index Adapters Pooling Guide Documentation
カスタムプロトコルセレクタ
Generates customized, end-to-end instructions
すべて AmpliSeq for Illumina Comprehensive Cancer Panel Support
MiSeq sequencing reagents in pre-filled, ready-to-use cartridges. Micro and nano formats are available for low output applications.
iSeq 100 Reagents feature a ready-to-use cartridge and one-channel SBS technology for simple, low-cost sequencing.
Expert-defined content targeting 113 genes associated with genetic cancer risk predisposition.
AmpliSeq for Illuminaのライブラリー調製とインデックスアダプターを使用して、イルミナシーケンサー用のアンプリコンライブラリーを迅速に作製します。
