Illumina DNA Prep offers flexibility for many whole-genome sequencing applications.
Illumina DNA Prep uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.
The Illumina DNA Prep workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*.
Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.
While accommodating various study requirements, the Illumina DNA Prep workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducible sequencing data.
The IDT for Illumina - DNA/RNA UD Indexes Sets A, B, C, and D offer up to 384 unique dual indexes, enabling accurate assignment of reads and efficient use of the flow cell. (Sets A and B are available now. Sets C and D are coming soon.) These unique dual index codes use 10 base pair codes. This change in base pair index codes require adjustments to the sequencing run setup.
Nextera DNA CD Indexes support up to 96-sample combinatorial dual indexing. The 24 CD Indexes are supplied in a tube format, and 96 in a plate format.
For whole-genome sequencing, Illumina DNA Prep is the recommended replacement for the Nextera DNA Library Prep Kit, which has been discontinued. If you were using Nextera DNA (Cat. No. FC-121-1030) or stand-alone components (Cat. Nos. 15027865 and 15027866) for ATAC-Seq** or other custom applications, the Illumina Tagment DNA TDE1 Enzyme and Buffer Kit is our suggested alternative.
*Demonstrated protocols available.
**Customer-reported application
Discover the power that Illumina library prep tagmentation technology can bring to your lab through this interactive experience. Our library prep solutions offer whole-genome sequencing across various applications, flexibility across Illumina sequencers, and integrated sample input for blood and saliva without quantification.
View Infographic
| 装置 | 推奨サンプル数 | リード長 |
|---|---|---|
| NextSeq 550 System | 1 sample per run (high output; based on 30× coverage of a human genome) | Up to 2 × 150 bp |
| NovaSeq 6000 System | 2–10 samples per run (dual flow cell; based on 30× coverage of a human genome) | Up to 2 × 125 bp (rapid run) Up to 2 × 150 bp (high output) |
| Illumina DNA Prep | TruSeq DNA PCR-Free | TruSeq DNA Nano | |
|---|---|---|---|
| Assay Time | ~3-4 hours (from DNA extraction to normalized library) | 5 hours total assay time | ~6 hours total assay time |
| Automation Capability | Liquid Handling Robots | Liquid Handling Robots | Liquid Handling Robots |
| Hands-On Time | 1-1.5 hours | 4 hours | ~4 hours |
| Input Quantity | Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. | 1 ug DNA | 100 ng genomic DNA |
| Mechanism of Action | Bead-linked transposome | Mechanical DNA fragmentation and adapter ligation. Workflow is PCR-free and gel-free. | Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free. |
| Method | Amplicon Sequencing, De Novo Sequencing, Shotgun Sequencing, Whole-Genome Sequencing | Genotyping by Sequencing, Shotgun Sequencing, Whole-Genome Sequencing | Genotyping by Sequencing, Shotgun Sequencing, Whole-Genome Sequencing |
| Specialized Sample Types | Blood, Saliva | Not FFPE-Compatible | Low-Input Samples, Not FFPE-Compatible |
| Species Category | Any Species, Bacteria, Drosophila, Human, Mammalian, Mouse, Nematode, Plant, Rat, Virus, Yeast, Zebrafish | Human, Mammalian, Mouse, Other, Plant, Rat | Human, Mammalian, Mouse, Other, Plant, Rat |
(A) Bead-linked transposomes mediate the simultaneous fragmentation of gDNA and the addition of Illumina sequencing primers. (B) Reduced-cycle PCR amplification amplifies sequencing ready DNA fragments and adds indexes and adapters. (C) Sequencing-ready fragments are washed and pooled.
Application Note | PDF 3 MB
An automated Nextera DNA Flex library preparation workflow for high-throughput metagenomics
Application Note | PDF < 1 MB
Nextera Crude Lysate Protocol for Metagenomic Whole-Genome Sequencing Studies
Application Note | PDF < 1 MB
Human Whole-Genome Sequencing with the Nextera DNA Flex Library Preparation Kit
Application Note | PDF < 1 MB
Direct Bacterial Colony Sequencing with the Nextera™ DNA Flex Library Preparation Kit
Application Note | PDF 1 MB
Microbial Whole-Genome Sequencing with the Nextera DNA Flex Library Preparation Kit
Application Note | PDF < 1 MB
Fast, Flexible, and Self-Normalizing Library Prep with Nextera Flex Chemistry
Application Note | HTML
Nextera DNA Flex Library Preparation for Soil Shotgun Metagenomics Analysis
Application Note | HTML
Data Sheet | PDF | 5 versions
Data Sheet | PDF | 5 versions
Illumina library preparation solutions
Brochure | PDF 2 MB
Illumina DNA PCR-Free Prep, Tagmentation
Data Sheet | PDF < 1 MB
Index Adapters Pooling Guide Documentation
Illumina DNA Prep Consumables & Equipment List Documentation
Trim Adapters for Nextera DNA Flex in Local Run Manager for MiniSeq Documentation
Illumina DNA Prep Support Documentation
Illumina DNA Prep Reference Guide Documentation
Illumina DNA Prep Checklist Documentation
カスタムプロトコルセレクタ
Generates customized, end-to-end instructions
少量のDNAインプットで、小さなゲノム、PCRアンプリコン、およびプラスミドのシーケンスライブラリーを90分以内で調製できます。
Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA.
Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes.
Nextera DNA Flex Library Prep Kit 選択ツール
