We recommend ordering the new Illumina DNA/RNA UD Indexes Sets A-D (20091654, 20091656, 20091658, 20091660). The IDT for Illumina DNA/RNA UD Indexes Sets A-D (20027213, 20027214,20042666, 20042667) are obsolete and the last order date is January 30, 2025, as announced in PON2023-1440.
Illumina DNA PCR-Free Prep Training
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Illumina DNA PCR-Free Library Prep offers flexibility for many whole-genome sequencing applications.
Save Time and Resources
The Illumina DNA PCR-Free Library Prep Kit uses a fast, user-friendly workflow. On-Bead Tagmentation chemistry reduces total library prep time to ~1.5 hours, from DNA extraction to library normalization.
Simplify Lab Operations
The Illumina DNA PCR-Free workflow supports a broad DNA input range (25 ng to 300 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots.
You can use liquid-handling robots to automate workflows for minimal touch points and significant time savings.
Obtain Reliable Results
While accommodating various study requirements, the Illumina DNA PCR-Free workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, delivering accurate base calling and variant identification for a broad range of DNA input amounts.
Access Flexible Throughput Options
The IDT for Illumina - DNA/RNA UD Indexes Sets A, B, C, and D Indexes offer up to 384 unique dual indexing that enables accurate assignment of reads and efficient use of the flow cell. (Sets A and B are available now; Sets C and D are coming soon.) These unique dual index codes use 10 base pair codes. This change in base pair index codes require adjustments to the sequencing run setup.
Fast, Automation-friendly Workflow
Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.
| Illumina DNA PCR-Free Prep | TruSeq DNA PCR-Free | TruSeq DNA Nano |
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Dr. Pietro Cattaneo and Dr. Cédric Howald from the Health 2030 Genome Center discuss their experience incorporating IGA and Illumina DNA PCR-Free Prep in their workflows and how deploying these solutions has enabled them to meet the demands of their large-scale WGS projects.
Read InterviewAn efficient solution for preparing and indexing sample libraries.
An efficient solution for preparing and indexing sample libraries.
The Illumina DNA PCR-Free Library Prep Kit provides superior read coverage across the GC-rich promoter region of the human RNPEPL1 gene, as compared to TruSeq DNA PCR-Free and TruSeq DNA Nano Library Prep Kits. Read maps were visualized with the Integrative Genomics Viewer (IGV) App, available in BaseSpace Sequence Hub.
Illumina DNA PCR-Free libraries prepared from a range of DNA inputs demonstrate (A) passing quality specifications for all DNA inputs and (B) equivalent callability performance. Q30 score = an inferred base call accuracy of 99.9%, autosome callability = the percentage of non-N reference positions in autosomal chromosomes with a passing genotype call, exon callability = the percentage of non-N reference positions in exons with a passing genotype call, SNPs = single nucleotide polymorphisms, indel = insertion-deletion mutation, precision (accuracy) = calculated as the ratio of [# of True Positive Calls/(# of True Positive Calls + # of False Positive Calls)], recall (sensitivity) = calculated as the ratio of [# of True Positive Calls/(# of True Positive Calls + # of False Negative Calls)]. Note: the lower limit sample input specifications for Illumina DNA PCR-Free have not been finalized.
Microbial whole-genome sequencing with Illumina DNA PCR-Free Prep, Tagmentation
Application Note | PDF < 1 MB
High-performance whole-genome sequencing with Illumina DNA PCR-Free Prep, Tagmentation
Application Note | PDF < 1 MB
Tunable insert sizes with Illumina DNA PCR-Free Prep, Tagmentation
Application Note | PDF < 1 MB
Illumina library preparation solutions
Brochure | PDF 2 MB
Illumina Genomics Architecture enables PopGen studies with Illumina DNA PCR-Free Prep
Application Note | PDF 2 MB
Optimal loading concentrations for Illumina DNA PCR-Free libraries
Technical Note | PDF < 1 MB
Balancing sample coverage for whole-genome sequencing
Technical Note | PDF < 1 MB
Illumina DNA PCR-Free Prep index correction
product_file | CSV < 1 MB
Illumina DNA PCR-Free Prep, Tagmentation
Data Sheet | PDF < 1 MB
Illumina Adapter Sequences Documentation
カスタムプロトコルセレクタ
Generates customized, end-to-end instructions
すべて Illumina DNA RNA UD Indexes Support すべて Illumina DNA PCR-Free Support
コーディングおよびノンコーディングトランスクリプトーム研究用の広範な種類のサンプルから迅速にライブラリーを調製して、非常に優れた柔軟性を持って研究を行うことができます。
包括的で迅速なターゲットエンリッチメントシーケンスにより、SARS-CoV-2株を含む一般的な呼吸器系ウイルスの高感度な検出と特性評価を行います。
