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Sample Multiplexing

Processing more samples in less time

Increase the number of samples sequenced per run and optimize high-throughput sequencing

Sample Multiplexing Overview

Sample multiplexing, also known as multiplex sequencing, allows large numbers of libraries to be pooled and sequenced simultaneously during a single run on Illumina instruments. Sample multiplexing is useful when targeting specific genomic regions or working with smaller genomes. Pooling samples exponentially increases the number of samples analyzed in a single run, without drastically increasing cost or time.

Individual "barcode" sequences are added to each DNA fragment during next-generation sequencing (NGS) library preparation so that each read can be identified and sorted before the final data analysis. These barcodes, or index adapters, can follow one of two major indexing strategies depending on your library prep kit and application..

Conceptual Overview of Sample Multiplexing

Multiplex Sequencing Highlights

  • Fast High-Throughput Strategy: Large sample numbers can be simultaneously sequenced during a single experiment
  • Cost-Effective Method: Sample pooling improves productivity by reducing time and reagent use
  • Simplified Analysis: Automatic sample identification with "barcodes" using Illumina data analysis software
Sample Multiplexing Facilitates Microbial Studies

Researchers leverage the speed and multiplexing capabilities of Illumina sequencers to identify microbes along the Mississippi River.

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Chan Zuckerberg Biohub and the NovaSeq System

Researchers at the Chan Zuckerberg Biohub are working to conduct innovative experiments and form new collaborations in genomics. The NovaSeq System enables them to bundle many samples together for medical research that may help to improve human health.

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Chan Zuckerberg Biohub and the NovaSeq System

Related Information

  • Minimizing Index Hopping: Find tips and best practices to avoid sequencing read misalignment when using sample multiplexing.
  • High-Throughput Library Prep Automation: Learn about automated liquid handling solutions designed to help labs prepare large quantities of sequencing libraries.
  • Consulting Services: Whether you are newly applying NGS or ramping up high-throughput sequencing, our highly experienced global network is here to help.
Flexible High-Throughput Sequencer

The NovaSeq 6000 System offers scalable throughput and flexibility for virtually any genome, sequencing method, and scale of project.

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