AmpliSeq for Illumina TCR beta-SR (Short Read) Panel is a highly multiplexed targeted resequencing panel intended for use with multiple sample types, including formalin-fixed paraffin-embedded (FFPE) tumor samples. It is designed to measure T cell diversity and clonal expansion by sequencing T cell receptor (TCR) beta chain rearrangements. You can use a single pool of multiplex PCR primers, library reagents, and sample barcodes to generate libraries from DNA/RNA extracted from FFPE, whole blood, fresh-frozen tissue, or fluorescence activated cell sorting (FACS)-sorted cells.
Key features include:
- High-Value Content
Obtain comprehensive coverage of TCR beta chain sequences
- Simple, Streamlined Workflow
Prepare sequencing-ready libraries in a single day from FFPE, whole blood, fresh/frozen tissue, or fluorescence-activated cell sorting (FACS) cells
- Low Input Requirement
Generate high-quality sequencing libraries from as little as 10 ng of input RNA
The AmpliSeq for Illumina TCR beta-SR Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels. It enables comprehensive characterization of all three hypervariable complementary determining regions (CDRs) of the TCR beta chain.
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