Optimal Cluster Density Best Practices

2020/02/14

In this video, we will discuss optimal cluster density and how over and under-clustering can affect your sequencing data. We will then examine common clustering issues and ways to prevent them. More resources: The Cluster Optimization Overview Guide: https://support.illumina.com/downloads/cluster-optimization-overview-guide-1000000071511.html Support Bulletins: “What is nucleotide diversity and why is it important?”: https://support.illumina.com/bulletins/2016/07/what-is-nucleotide-diversity-and-why-is-it-important.html “Best practices for low diversity sequencing on the NextSeq and MiniSeq systems.”: https://support.illumina.com/bulletins/2016/03/best-practices-for-lowdiversity-sequencing-on-the-nextseq-and-miniseq-systems.html “How much PhiX spike-in is recommended when sequencing low diversity libraries on Illumina platforms?”: https://support.illumina.com/bulletins/2017/02/how-much-phix-spike-in-is-recommended-when-sequencing-low-divers.html Video Tips: Diagnosing Suboptimal Clustering in Nonpatterned Flow Cells: https://www.youtube.com/watch?v=PDn4Q9sRgSU&feature=youtu.be Diagnosing Suboptimal Clustering in Patterned Flow Cells: https://www.youtube.com/watch?v=jp9fNcR3O20&feature=youtu.be “How do I optimize Amplicon Sequencing Data part 1”: https://www.youtube.com/embed/s79pgik8XbA?autoplay=1&rel=0 “How do I optimize Amplicon Sequencing Data part 2”: https://www.youtube.com/embed/fprcw5YRi9M?autoplay=1&rel=0 Tech notes: Low diversity Sequencing on MiSeq: https://www.illumina.com/documents/products/technotes/technote_low_diversity_rta.pdf Low diversity Sequencing on HiSeq: https://www.illumina.com/documents/products/technotes/technote-hiseq-low-diversity.pdf Presenters: Tim Gilmartin, Staff Field Applications Scientist, Illumina Michelle Ye, Staff Technical Applications Scientist, Illumina. Disclaimer: For Research Use Only. Not for use in diagnostic procedures. Subscribe to the Illumina video channel http://www.youtube.com/subscription_center?add_user=IlluminaInc A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Illumina technology is responsible for generating more than 90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. *Data calculations on file. Illumina, Inc., 2015. View customer spotlight videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View Illumina webinars https://www.youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View Illumina product videos https://www.youtube.com/playlist?list=PLKRu7cmBQlaj6YuZmkfxZcT9twqDgP2Xd View Illumina support videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM

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