Nextera DNA

Nextera DNA

Nextera DNA Library Preparation Kits provide the fastest and easiest workflow, enabling sequencing-ready libraries to be generated in less than 90 minutes, with less than 15 minutes of hands-on time. DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment.

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* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Based on 30x coverage of a human genome.
Project Recommendations
Species Compatibility Compatible with most large DNA genomes
Recommended Illumina Instrument* NextSeq 500 HiSeq 2500 (Dual Flow Cell)
Recommended Samples per run 1 2-10
Run Mode Mid Output High Output Rapid Run High Output
Max Recommended Read Length Up to 2x150 bp Up to 2x150 bp Up to 2x150 bp Up to 2x125 bp
Recommended Secondary Analysis
  • BWA WGS BaseSpace App
  • Isaac WGS v2 BaseSpace App
  • SPAdes Genome Assembler
Recommended Down Stream Analysis VariantStudio