TruSeq Amplicon Cancer Panel
TruSeq Amplicon Cancer Panel
The TruSeq Amplicon Cancer Panel (TSACP) product includes an Illumina validated oligo pool that generates 212 amplicons targeting mutational hotspots from 48 genes associated with both solid and hematological cancers.
TruSeq Amplicon Cancer Panel Gene List
ABL1 AKT1CTNNB1 ALK
EGFR APC
ERBB2 ATM BRAF CDH1 CDKN2A CSF1R ERBB4 FBXW7
HNF1A FGFR1
HRAS FGFR2
IDH1 FGFR3 FLT3 GNA11 GNAQ GNAS JAK2 JAK3
NPM1 KDR
NRAS KIT
PDGFRA KRAS MET MLH1 MPL NOTCH1 PIK3CA PTEN
STK11 PTPN11
TP53 RB1
VHL RET SMAD4 SMARCB1 SMO SRC
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| * Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application. † Based on ~1,000x average coverage. |
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| Project Recommendations | ||||
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| Species Compatibility | Human | |||
| Recommended Illumina Instrument* | MiSeq | NextSeq 500 | ||
| Recommended Samples per run† | 42 (v2 chemistry) | 96 | 96 | |
| Run Mode | - | Mid Output | High Output | |
| Max Recommended Read Length | Up to 2x150 bp | Up to 2x150 bp | Up to 2x150 bp | |
| Recommended Secondary Analysis |
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| Recommended Down Stream Analysis | VariantStudio | VariantStudio | ||