Nextera DNA Exome
Nextera DNA Exome
The most efficient Exome workflow focused on 45 Mb of coding sequence from >98% of RefSeq, CCDS, and Ensembl coding content.
| Catalog Number | Total Samples | Number of Enrichments | Indexing |
|---|---|---|---|
| 20020616 | 24 | 8 | 24 combinatorial dual indexes |
| 20020617 | 96 | 8 | 96 combinatorial dual indexes |
| Library Preparation for Enrichment | |||
|---|---|---|---|
| Catalog Number | Total Samples | ||
| 20020187 | 24 | ||
| 20020188 | 96 | ||
| Index Primers | |||
| Catalog Number | Total Samples | Indexing | |
| 20018707 | 24 | 24 combinatorial dual indexes | |
| 20018708 | 96 | 96 combinatorial dual indexes | |
| * When choosing your own components, Panel and Enrichment Reagents can be purchased through IDT | |||
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| Species Compatibility | Human | ||||||
|---|---|---|---|---|---|---|---|
| Recommended Illumina Instrument | NextSeq 500 | HiSeq 2500 (Dual Flow Cell) | NovaSeq 6000 (Dual Flow Cell)† | ||||
| Recommended Samples per run‡ | 3 | 12 | 24 | 156 | 96 | 96 | 192 |
| Run Mode | Mid Output | High Output | Rapid Run | High Output | S1§ | S2 | S4 |
| Max Recommended Read Length | 2×75 bp | 2×75 bp | 2×75 bp | 2×75 bp | ≤ 2×100 bp | ||
| Recommended Secondary Analysis |
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| Recommended Down Stream Analysis | VariantStudio | ||||||
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* Library prep is compatible with all Illumina instruments, except the X5 and X10 systems. Recommended instruments chosen based on typical output and analysis need for selected application. † Limited by index combinations (HT). ‡ 50M Reads, 50× Coverage, 4GB of Data. § NovaSeq S1 Reagent Kits are not currently available. |
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