Comparison Table
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| TruSight Cancer | TruSeq Amplicon Cancer Panel | TruSight Tumor 15 | TruSight Tumor 26 | TruSight Myeloid | TruSight Tumor 170 | |
|---|---|---|---|---|---|---|
| Key Use | Germline mutation detection for common and rare cancers. | Somatic mutational hotspots in a broad spectrum of cancers. | Focused panel to assess relevant solid tumor somatic variants in a simple, sample-to-data workflow | Somatic mutation detection in solid tumors. | Somatic mutation detection in myeloid malignancies. | Comprehensive somatic mutation detection in solid tumors. Uses variant calling information from both DNA and RNA. |
| Design | Fixed set of probes that enrich for 94 genes and 284 SNPs associated with common and rare cancers. | Fixed set of probes that amplify 212 amplicons from mutational hotspots in 48 cancer- associated genes. | Fixed set of oligos that amplify 250 amplicons from 15 genes associated with solid tumors | Fixed set of probes that amplify 174 amplicons from tumor suppressor genes and mutational hotspots in 26 genes associated with solid tumors. | Fixed set of probes that amplify 568 amplicons from tumor suppressor genes and mutational hotspots. Targets 54 full or partial genes associated with myeloid malignancies. | Fixed set of probes that enrich for full coding sequences of 170 genes. Single nucleotide variants, small insertions, and deletions are called in 151 genes; amplifications are called in 59 genes; fusions and splice variants are called in 55 genes. |
| Library prep time | 12 hours | 7 hours | 7.5 hours | 11 hours | 8 hours | 32 hours |
| Genomic Content | 225 kb (94 genes, 284 SNPs) | >35 kb (212 amplicons, 48 genes) | 44kb (250 amplicons, 15 genes) | 21kb (174 amplicons, 26 genes) | 141kb (568 amplicons, 54 genes) | 533 kb DNA 358 kb RNA |
| Input Amount | 50ng gDNA | 150–250ng gDNA 250ng FFPE gDNA | 20ng FFPE gDNA | 30-300ng FFPE gDNA (based off QC assay) | 50ng gDNA | 40–120 ng FFPE DNA 40–80 ng FFPE RNA |
| FFPE Compatibility | No | Yes | Yes | Yes | No | Yes |
| Multiplexing | Up to 96plex | Up to 96plex | 1–24plex | Up to 48plex | Up to 96plex | Up to 32plex for DNA using both index sets, up to 16plex for RNA |
| Methodology | Enrichment | Amplicon | mPCR Amplicon | Amplicon | Amplicon | Enrichment |