Comparison Table
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| Nextera XT | Nextera DNA Flex | |
|---|---|---|
| Intended Use | Allows the preparation of sequencing-ready libraries for small genomes, amplicons, and plasmids in less than 90 minutes with only 15 minutes of hands-on time. Amplicon inputs are required be at least 300 bp in length. | Illumina’s fastest and easiest workflow from sample to normalized library. |
| Input Quantity | 1ng | 1–500ng |
| Assay Time | ~1.5 hours | 3–3.5 hours (from blood/saliva DNA extraction to normalized library) |
| Multiplexing | 384 dual index | 96 dual index |
| Fragmentation | Enzymatic | Enzymatic |
| Target Insert Size | 300bp–1.5kb | ~500bp |
| Bisulfite Compatibility | No | No |