Ampliseq for Illumina Immune Response Panel

Ampliseq for Illumina Immune Response Panel

AmpliSeq for Illumina Immune Response Panel is a targeted resequencing assay for quantitating expression of cancer biomarkers in 395 genes involved in tumor–immune system interactions. The Immune Response panel is part of an integrated workflow that includes AmpliSeq for Illumina PCR-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis. Starting with as little as 10 ng input RNA, the panel enables detection of biomarkers associated with different leukocyte subsets, antigen presentation, checkpoint pathways, and tumor progression. The low-input requirement enables use with samples of varying quality, including formalin-fixed, paraffin-embedded (FFPE) tissues.

Panel:
20019169 AmpliSeq for Illumina Immune Response Panel, 24 Reactions
Library Prep:
20019101 AmpliSeq for Illumina Library PLUS, 24 Reactions
20019102 AmpliSeq for Illumina Library PLUS, 96 Reactions
20019103 AmpliSeq for Illumina Library PLUS, 384 Reactions
Indexes:
20019105 AmpliSeq for Illumina CD Indexes Set A, 96 Indexes, 96 Reactions
Project Recommendations
Species Compatibility Human
Recommended Illumina Instrument* MiniSeq MiSeq iSeq 100
Recommended Samples per run Mid Output High Output v2 Micro v2 v3 4
8 24 4 15 24
Max Recommended Read Length 2×150
Recommended Secondary Analysis RNA Amplicon Module, RNA Amplicon App
Recommended Down Stream Analysis N/A

* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.

† Assumes 1M reads per sample

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