AmpliSeq for Illumina Immune Response Panel is a targeted resequencing assay for quantitating expression of cancer biomarkers in 395 genes involved in tumor–immune system interactions. The Immune Response panel is part of an integrated workflow that includes AmpliSeq for Illumina PCR-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis. Starting with as little as 10 ng input RNA, the panel enables detection of biomarkers associated with different leukocyte subsets, antigen presentation, checkpoint pathways, and tumor progression. The low-input requirement enables use with samples of varying quality, including formalin-fixed, paraffin-embedded (FFPE) tissues.
| Species Compatibility | Human | |||||
|---|---|---|---|---|---|---|
| Recommended Illumina Instrument* | MiniSeq | MiSeq | iSeq 100 | |||
| Recommended Samples per run† | Mid Output | High Output | v2 Micro | v2 | v3 | 4 |
| 8 | 24 | 4 | 15 | 24 | ||
| Max Recommended Read Length | 2×150 | |||||
| Recommended Secondary Analysis | RNA Amplicon Module, RNA Amplicon App | |||||
| Recommended Down Stream Analysis | N/A | |||||
* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Assumes 1M reads per sample
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