Nextera Mate Pair Library Preparation Kitは、de novoシーケンス、ゲノムフィニッシング、および構造変異の検出などに理想的なアプローチです。ゲノムサイズの大きいDNAにも使用することができ、がん細胞由来のサンプルなど非常に複雑なゲノムにも対応しています。
ゲルフリーとゲル切りありの両方のプロトコールをご用意しています：
このキットでは、検出可能なジャンクション配列が得られるため、メイトペアの断片の両方を高い精度で確認できます。
Nextera Mate Pair KitではTruSeq DNA Library Preparationのマスターミックス試薬を使用しているため、アッセイステップが少なく、操作時間を短縮します。
With the Nextera Mate Pair kit, researchers generated up to 13 kb insert-size libraries for de novo sequencing of the kiwi genome on the HiSeq System.
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Using a combination of short and long insert sizes with paired-end sequencing results in maximal coverage of the genome for de novo assembly. Because larger inserts can pair reads across greater distances, they provide a better ability to read through highly repetitive sequences and regions where large structural rearrangements have occured. Shorter inserts sequenced at higher depths can fill in gaps missed by larger inserts sequenced at lower depths. Thus a diverse library of short and long inserts results in better de novo assembly, leading to fewer gaps, larger contigs, and greater accuracy of the final consensus sequence.
Nextera Library Validation and Cluster Density Optimization
Technical Note | PDF< 1 MB
Data Processing of Nextera Mate Pair Reads on Illumina Sequencing Platforms
Technical Note | PDF1 MB
De Novo Assembly of Small Genome Nextera Mate Pair Libraries with a Single MiSeq System Run
Application Note | PDF< 1 MB
De Novo Assembly of Bacterial Genomes
Application Note | PDF< 1 MB
Nextera Mate Pair Library Preparation Kit
Data Sheet | PDF< 1 MB
Nextera Mate Pair Library Preparation Kit Support Documentation
Illumina Experiment Manager User Guide Documentation
Nextera DNA Sample Preparation Product Insert Documentation
カスタムプロトコルセレクタ
Generates customized, end-to-end instructions
