G&T-Seq
G&T-Seq
Genome and transcriptome sequencing (G&T-Seq) is a protocol that can separate and sequence genomic DNA and full-length mRNA from single cells. In this method, single cells are isolated and lysed. RNA is captured using biotinylated oligo-dT capture primers and separated from DNA using Streptavidin coated magnetic beads. Smart-seq2 method is used to amplify captured RNA on the bead, while MDA method is used to amplify DNA. After sequencing, integrating DNA and RNA sequences provides insights into gene expression profile of single cells.