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Depletion of ribosomal RNA (rRNA) prior to RNA sequencing (RNA-Seq) enables researchers to focus on analyzing high-value portions of the transcriptome. Illumina Ribo-Zero rRNA Removal Kits and Globin-Zero rRNA/globin mRNA Removal Kits set the standard for rRNA and globin mRNA depletion for RNA-Seq library preparation.
Ribo-Zero treated and Globin-Zero treated RNA can be used to make whole-transcriptome RNA-Seq libraries using the ScriptSeq v2 RNA-Seq Library Preparation kit.
The Ribo-Zero and Globin-Zero Kits:
| RNA Sample | Kit | Removes Euk. Cytoplasmic rRNA | Removes Mitochondrial rRNA | Removes Bacterial rRNA | Removes Chloroplast rRNA | Removes Globin mRNA |
|---|---|---|---|---|---|---|
| Human and other animal species and FFPE RNA | Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) | ✓ | ||||
| Human and other animal species; FFPE RNA | Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) | ✓ | ✓ | |||
| Human microbiome and other bacteria-infected human, mouse, rat samples | Ribo-Zero Gold rRNA Removal Kit (Epidemiology) | ✓ | ✓ | ✓ | ||
| Bacteria (mixed populations of gram-negative and gram-positive bacteria) |
Ribo-Zero rRNA Removal Kit (Bacteria) | ✓ | ||||
| Gram-negative bacteria | Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria) | ✓ | ||||
| Gram-positive bacteria | Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) | ✓ | ||||
| Plant leaf tissue | Ribo-Zero rRNA Removal Kit (Plant Leaf) | ✓ | ✓ | ✓ | ||
| Plant seed or root tissue | Ribo-Zero rRNA Removal Kit (Plant Seed/Root) | ✓ | ✓ | ✓ | ||
| Mammalian blood RNA | Globin-Zero Gold Kit | ✓ | ✓ | ✓ | ||
| Yeast | Ribo-Zero rRNA Removal Kit (Yeast) | ✓ | ✓ |