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The product configuration for Cat. No. RS-122-2501, RS-122-2502, and RS-122-2503 has changed. In the new configuration, the library preparation and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.
TruSeq Stranded Total RNA with Ribo-Zero Globinキットは、迅速かつ効率的なシーケンスライブラリー調製ワークフローにより、血液由来RNAからトランスクリプトームの全体像を明確に捉えることができます。
Ribo-ZeroのリボソームRNAを除去するケミストリーとRNAシーケンス（RNA-Seq）テクノロジーの2つの利点が統合されており、ヒト、マウス、またはラットのサンプルの全トランスクリプトーム解析ができるキットです。このテクノロジーにより実現すること：
血液由来RNAの全トランスクリプトーム解析では、リボソームRNA（細胞質とミトコンドリア両方）および全血中に高濃度で存在するグロビンmRNAという2種類の大量のRNAの除去が必要です。
従来の除去方法では2つの独立したステップが必要です。これは、より多くの試薬、長時間のワークフロー、より多くのRNAインプット量が必要であることを意味します。TruSeq Stranded Total RNA with Ribo-Zero Globinでは、Ribo-Zeroケミストリーを利用して、1つの短時間のステップにより2種類の大量のRNAを効率的に除去します。
| 装置 | 推奨サンプル数 | リード長 |
|---|---|---|
| NextSeq 550 System | RNA Profiling: 6-20 samples per run (based on 20 million reads per
sample) Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample) |
2 x 75 bp 2 x 75 bp |
| HiSeq 2500 System | RNA Profiling: 30-200 samples per run (dual flow cell; based on 20
million reads per sample) Transcriptome Analysis: 12-80 samples per run (dual flow cell; based on 50 million reads per sample) |
2 x 75 bp 2 x 75 bp |
| NovaSeq 6000 System | RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4:
768. Limited by index combinations (dual). Based on 20 million reads. Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads. |
≤ 2 × 100 bp ≤ 2 × 100 bp |
Technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2(FPKM). R2 value is shown.
TruSeq Stranded Total RNA gives excellent coverage across the top 1,000 expressed transcripts in both fresh-frozen (FF, top) and FFPE (bottom) tumor and matched normal breast tissue, with > 98% aligned stranded reads. X-axis: position along transcript, Y-axis = percent coverage of combined reads.
Data Sheet | PDF | 5 versions
Sequencing Library qPCR Quantification Guide Documentation
TruSeq Stranded Total RNA Checklist Documentation
TruSeq Stranded Total RNA Reference Guide Documentation
Illumina Experiment Manager User Guide Documentation
TruSeq HT Dual Index Quick Reference Card Documentation
TruSeq Stranded Total RNA Sample Preparation User-Supplied Consumables and Equipment Documentation
TruSeq Stranded Total RNA Consumables & Equipment Documentation
TruSeq Sample Prep Best Practices Guide Documentation
TruSeq Stranded Total RNA Sample Preparation LS EUC and LTF Documentation
TruSeq Stranded Total RNA Sample Prep HS EUC and LTF Documentation
TruSeq Stranded Total RNA Sample Prep Guide Documentation
TruSeq Stranded Total RNA Dual Index Product Insert Documentation
TruSeq Stranded Total RNA Documentation
カスタムプロトコルセレクタ
Generates customized, end-to-end instructions
A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
このRNA-Seqライブラリー調製キットは、植物の完全なトランスクリプトーム像を捉えます。植物の葉、種、根組織からrRNAを除去します。
Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.
20,000を超えるヒトRefSeq遺伝子の発現レベルを測定するターゲットパネル。
FFPE 組織やその他の品質の低いサンプルからのRNAのシーケンスに適した再現性の高い経済的なソリューションです。わずか10 ng の total RNA から精確なデータを得ることができます。
Limited Use Label License: The TruSeq Stranded Total RNA with Ribo-Zero Globin Workflow product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.
