The product previously known as the TruSeq Stranded mRNA Library Prep Kit (Cat. No. RS-122-2101, RS-122-2102, and RS-122-2103) is now called TruSeq Stranded mRNA. The product configuration has changed. In the new configuration, the library preparation and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.
TruSeq Stranded mRNA Library Prep Kit は、コーディングトランスクリプトーム解析のための効率的でコスト効率と拡張性の高いソリューションです。多種多様なサンプルに対応しています。
mRNA鎖の方向を正確に測定して、アンチセンス転写物を検出し、転写産物アノテーションとアライメント効率を向上させることができます。カバレッジの均一性が高いため、選択的転写産物、遺伝子融合、およびアリル特異的遺伝子発現などの特性を容易に発見できます。
ストランド情報により、二本鎖DNAのどちらにRNA転写産物が由来しているのかを特定することができます。この情報は、特にヒト以外のサンプルの場合に、転写産物アノテーションの信頼度を高めます。ストランドの由来を特定することでアライメントされるリードの割合が上がり、サンプルあたりのシーケンスコストを削減できます。
このキットは、標準的なサンプルも品質の低いサンプルも同じように厳格に調べることができ、ワークフローはさまざまな研究デザインに対応しています。
| 装置 | 推奨サンプル数 | リード長 |
|---|---|---|
| NextSeq 550 System | RNA Profiling: 13–40 samples per run (based on 10 million reads per
sample) Transcriptome Analysis: 5–16 samples per run (based on 25 million reads per sample) |
2 x 75 bp 2 x 75 bp |
| HiSeq 2500 System | RNA Profiling: 60–400 samples per run (dual flow cell; based on 10
million reads per sample) Transcriptome Analysis: 24–160 samples per run (dual flow cell; based on 25 million reads per sample) |
2 x 75 bp 2 x 75 bp |
| NovaSeq 6000 System | RNA Profiling (samples per run, dual flow cell): S1: 320, S2:384,
S4:768. Limited by index combinations (dual). Based on 10 million reads. Transcriptome Analysis (samples per run, dual flow cell): S1: 128, S2: 256, S4: 768. Limited by index combinations (dual). Based on 25 million reads. |
≤ 2 × 100 bp ≤ 2 × 100 bp |
Researchers at the Icahn School of Medicine at Mount Sinai are studying mtDNA, piRNA, and TCR repertoire diversity to unlock the secrets of cancer, infertility, and autoimmune disease.
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Figure 2 from TruSeq Stranded mRNA and Total RNA Library Prep Kits Data Sheet, December 2015
Technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2(FPKM). R2 value is shown.
Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
Application Note | HTML
Data Sheet | PDF | 5 versions
Sequencing Library qPCR Quantification Guide Documentation
TruSeq Stranded mRNA Checklist Documentation
TruSeq Stranded mRNA Documentation
TruSeq Stranded mRNA Dual Index Product Insert Documentation
Illumina Experiment Manager User Guide Documentation
TruSeq HT Dual Index Quick Reference Card Documentation
TruSeq Sample Prep Best Practices Guide Documentation
TruSeq Stranded mRNA Sample Prep HS EUC and LTF Documentation
TruSeq Stranded mRNA Sample Prep Guide Documentation
TruSeq Stranded mRNA Reference Guide Documentation
TruSeq Stranded mRNA Sample Prep LS EUC and LTF Documentation
TruSeq Stranded mRNA Sample Preparation User-Supplied Consumables and Equipment Documentation
TruSeq Stranded mRNA Consumables & Equipment Documentation
カスタムプロトコルセレクタ
Generates customized, end-to-end instructions
FFPE 組織やその他の品質の低いサンプルからのRNAのシーケンスに適した再現性の高い経済的なソリューションです。わずか10 ng の total RNA から精確なデータを得ることができます。
A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
単一ステップでrRNAおよびグロビンmRNAを除去する効率的なワークフローで、血液由来のRNAから全トランスクリプトームシーケンスライブラリーを調製します。
20,000を超えるヒトRefSeq遺伝子の発現レベルを測定するターゲットパネル。
This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERCIAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.
