The product previously known as the TruSeq Stranded mRNA Library Prep Kit (Cat. No. RS-122-2101, RS-122-2102, and RS-122-2103) is now called TruSeq Stranded mRNA. The product configuration has changed. In the new configuration, the library preparation and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.

TruSeq Stranded mRNA

Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information. Read More...
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Library Prep

TruSeq® Stranded mRNA Library Prep (48 Samples)

20020594

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TruSeq Stranded mRNA Library Prep (96 Samples)

20020595

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Index Adapters

TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples)

20020492

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TruSeq RNA Single Indexes Set B (12 Indexes, 48 Samples)

20020493

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TruSeq RNA CD Index Plate (96 Indexes, 96 Samples)

20019792

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IDT for Illumina – TruSeq RNA UD Indexes (24 Indexes, 96 Samples)

20020591

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IDT for Illumina – TruSeq RNA UD Indexes (96 Indexes, 96 Samples)

20022371

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アクセサリー製品

Illumina Free Adapter Blocking Reagent (12 reactions)

20024144

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Illumina Free Adapter Blocking Reagent (48 reactions)

20024145

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Product Highlights

TruSeq Stranded mRNA Library Prep Kit は、コーディングトランスクリプトーム解析のための効率的でコスト効率と拡張性の高いソリューションです。多種多様なサンプルに対応しています。

高い精度と正確度

mRNA鎖の方向を正確に測定して、アンチセンス転写物を検出し、転写産物アノテーションとアライメント効率を向上させることができます。カバレッジの均一性が高いため、選択的転写産物、遺伝子融合、およびアリル特異的遺伝子発現などの特性を容易に発見できます。

高いコスト効率

ストランド情報により、二本鎖DNAのどちらにRNA転写産物が由来しているのかを特定することができます。この情報は、特にヒト以外のサンプルの場合に、転写産物アノテーションの信頼度を高めます。ストランドの由来を特定することでアライメントされるリードの割合が上がり、サンプルあたりのシーケンスコストを削減できます。

拡張性

このキットは、標準的なサンプルも品質の低いサンプルも同じように厳格に調べることができ、ワークフローはさまざまな研究デザインに対応しています。

仕様

推奨プロジェクト

装置 推奨サンプル数 リード長
NextSeq 550 System RNA Profiling: 13–40 samples per run (based on 10 million reads per sample)
Transcriptome Analysis: 5–16 samples per run (based on 25 million reads per sample)
2 x 75 bp
2 x 75 bp
HiSeq 2500 System RNA Profiling: 60–400 samples per run (dual flow cell; based on 10 million reads per sample)
Transcriptome Analysis: 24–160 samples per run (dual flow cell; based on 25 million reads per sample)
2 x 75 bp
2 x 75 bp
NovaSeq 6000 System RNA Profiling (samples per run, dual flow cell): S1: 320, S2:384, S4:768. Limited by index combinations (dual). Based on 10 million reads.
Transcriptome Analysis (samples per run, dual flow cell): S1: 128, S2: 256, S4: 768. Limited by index combinations (dual). Based on 25 million reads. 
≤ 2 × 100 bp
≤ 2 × 100 bp

Method-Specific Workflow Example

 

Customer Stories

Unraveling Disease Mechanisms With The NextSeq 500 System

Researchers at the Icahn School of Medicine at Mount Sinai are studying mtDNA, piRNA, and TCR repertoire diversity to unlock the secrets of cancer, infertility, and autoimmune disease.

Read More
BRC-Seq Customers Benefit from Streamlined BaseSpace Integrated Workflow

BaseSpace enables tracking of samples, from library prep to data analysis, helping BRC-Seq deliver high-quality sequencing data and additional value to its customers.

Read More

Supporting Data and Figures

Technical Replicates
Supporting Data and Figures

Figure 2 from TruSeq Stranded mRNA and Total RNA Library Prep Kits Data Sheet, December 2015

Technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2(FPKM). R2 value is shown.

 
Find the Right Kit

Determine the best library prep kit or array for your needs based on your starting material and method of interest.

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Related Products

This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERCIAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.