|NextSeq 550 System||RNA Profiling: 13–40 samples per run (based on 10 million reads per
Transcriptome Analysis: 5–16 samples per run (based on 25 million reads per sample)
|2 x 75 bp
2 x 75 bp
|HiSeq 2500 System||RNA Profiling: 60–400 samples per run (dual flow cell; based on 10
million reads per sample)
Transcriptome Analysis: 24–160 samples per run (dual flow cell; based on 25 million reads per sample)
|2 x 75 bp
2 x 75 bp
|NovaSeq 6000 System||RNA Profiling (samples per run, dual flow cell): S1: 320, S2:384,
S4:768. Limited by index combinations (dual). Based on 10 million reads.
Transcriptome Analysis (samples per run, dual flow cell): S1: 128, S2: 256, S4: 768. Limited by index combinations (dual). Based on 25 million reads.
|≤ 2 × 100 bp
≤ 2 × 100 bp
Figure 2 from TruSeq Stranded mRNA and Total RNA Library Prep Kits Data Sheet, December 2015
Technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2(FPKM). R2 value is shown.
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