TruSeq RNA Exome

Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA. 続きを読む...
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Library Prep

TruSeq® RNA Library Prep for Enrichment (48 Samples)




TruSeq® RNA Enrichment (12 enrichments)



Index Adapters

TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples)



TruSeq RNA Single Indexes Set B (12 Indexes, 48 Samples)




Illumina Exome Panel – Enrichment Oligos Only



Illumina® Free Adapter Blocking Reagent (12 Reactions)



Illumina® Free Adapter Blocking Reagent (48 Reactions)




TruSeq RNA Exome, previously known as the TruSeq RNA Access Library Prep Kit, converts total RNA into template molecules of known strand origin, followed by sequence-specific capture of coding RNA. This provides a low-cost solution for analyzing human RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissues and other low-quality samples.

  • Affordability and focus—Isolating human transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth
  • High-quality data from difficult samples—Optimized for sequencing RNA from degraded samples, including FFPE tissues
  • Samples with limited starting material—Greatly reduced sample input requirements (as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples) while maintaining high sensitivity

TruSeq RNA Exome generates RNA sequencing (RNA-Seq) libraries from degraded samples that focus on the RNA coding regions. Isolating these high-value content regions to help maximize discovery power, while requiring only a fraction of the read depth of total RNA sequencing. The results are low input requirements, high sample throughput, and cost-effective transcriptome analysis. Reagent volumes supplied are sufficient to support 1- to 4-plex enrichment reactions.

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Read a comprehensive assessment of Illumina’s ribosomal RNA depletion protocol




装置 推奨サンプル数 リード長
NextSeq 550 System 5 to 16 samples per run (based on 25 million reads per sample) 2 x 75 bp
HiSeq 2500 System 24-160 samples per run (dual flow cell; based on 25 million reads per sample) 2 x 75 bp
NovaSeq 6000 System Samples per run (dual flow cell): S1: 96, S2: 96, S4: 192 (currently limited by available index combinations) 2 × 100 bp


TruSeq RNA Exome Illumina RNA Prep with Enrichment Illumina Stranded mRNA Prep
Automation Capability Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots
Method Exome Sequencing, mRNA Sequencing mRNA Sequencing, Target Enrichment, Target Enrichment, Targeted RNA Sequencing mRNA Sequencing
Specialized Sample Types FFPE Tissue, Low-Input Samples Blood, FFPE Tissue, Low-Input Samples Low-Input Samples, Not FFPE-Compatible
Species Category Human Human, Virus Bovine, Human, Mammalian, Mouse, Rat

The product previously known as the TruSeq RNA Access Library Prep Kit (Cat. No. RS-301-2001 and RS-301-2002) is now called TruSeq RNA Exome. The product configuration has changed. In the new configuration, major components such as library preparation, index adapters, enrichment reagents, and exome panels can be purchased separately. If you were previously buying Cat. No. RS-301-2001 or Cat. No. RS-301-2002, purchase the following components: TruSeq RNA Library Prep for Enrichment (Cat. No. 20020189), TruSeq RNA Enrichment (Cat. No. 20020490), Exome Panel (Cat. No. 20020183), and one item from the Index Adapters section below.

Method-Specific Workflow Example


Customer Stories

Supporting Data and Figures

Efficient Gene Fusion Discovery
Supporting Data and Figures

TruSeq RNA Exome enables detection of expressed fusion transcripts without the need to design probes specific for the fusion junction. The well-characterized BCR-ABL fusion is detected efficiently in the Universal Human Reference RNA (UHRR) sample at 25 M reads.


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