TruSeq RNA Exome, previously known as the TruSeq RNA Access Library Prep Kit, converts total RNA into template molecules of known strand origin, followed by sequence-specific capture of coding RNA. This provides a low-cost solution for analyzing human RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissues and other low-quality samples.
TruSeq RNA Exome generates RNA sequencing (RNA-Seq) libraries from degraded samples that focus on the RNA coding regions. Isolating these high-value content regions to help maximize discovery power, while requiring only a fraction of the read depth of total RNA sequencing. The results are low input requirements, high sample throughput, and cost-effective transcriptome analysis. Reagent volumes supplied are sufficient to support 1- to 4-plex enrichment reactions.
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Read a comprehensive assessment of Illumina’s ribosomal RNA depletion protocol
| 装置 | 推奨サンプル数 | リード長 |
|---|---|---|
| NextSeq 550 System | 5 to 16 samples per run (based on 25 million reads per sample) | 2 x 75 bp |
| HiSeq 2500 System | 24-160 samples per run (dual flow cell; based on 25 million reads per sample) | 2 x 75 bp |
| NovaSeq 6000 System | Samples per run (dual flow cell): S1: 96, S2: 96, S4: 192 (currently limited by available index combinations) | 2 × 100 bp |
| TruSeq RNA Exome | Illumina RNA Prep with Enrichment | Illumina Stranded mRNA Prep | |
|---|---|---|---|
| Automation Capability | Liquid Handling Robots | Liquid Handling Robots | Liquid Handling Robots |
| Method | Exome Sequencing, mRNA Sequencing | mRNA Sequencing, Target Enrichment, Target Enrichment, Targeted RNA Sequencing | mRNA Sequencing |
| Specialized Sample Types | FFPE Tissue, Low-Input Samples | Blood, FFPE Tissue, Low-Input Samples | Low-Input Samples, Not FFPE-Compatible |
| Species Category | Human | Human, Virus | Bovine, Human, Mammalian, Mouse, Rat |
The product previously known as the TruSeq RNA Access Library Prep Kit (Cat. No. RS-301-2001 and RS-301-2002) is now called TruSeq RNA Exome. The product configuration has changed. In the new configuration, major components such as library preparation, index adapters, enrichment reagents, and exome panels can be purchased separately. If you were previously buying Cat. No. RS-301-2001 or Cat. No. RS-301-2002, purchase the following components: TruSeq RNA Library Prep for Enrichment (Cat. No. 20020189), TruSeq RNA Enrichment (Cat. No. 20020490), Exome Panel (Cat. No. 20020183), and one item from the Index Adapters section below.
TruSeq RNA Exome enables detection of expressed fusion transcripts without the need to design probes specific for the fusion junction. The well-characterized BCR-ABL fusion is detected efficiently in the Universal Human Reference RNA (UHRR) sample at 25 M reads.
Data Sheet | PDF | 5 versions
RNA-Seq Offers Significant Advantages in Immuno-Oncology Research
White Paper | PDF 2 MB
Evaluating RNA Quality from FFPE Samples
Technical Note | PDF 1 MB
Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
Application Note | HTML
TruSeq RNA Exome Checklist Documentation
TruSeq RNA Exome Documentation
TruSeq RNA Exome Consumables & Equipment Documentation
TruSeq RNA Exome Reference Guide Documentation
カスタムプロトコルセレクタ
Generates customized, end-to-end instructions
ヒト、マウス、ホルマリン固定パラフィン包埋（FFPE）組織を含むさまざまな種やサンプルタイプに対応する、堅牢で拡張性の高い全トランスクリプトーム解析（RNA-Seq）ソリューションです。
