The product configuration for Cat. No. RS-122-2401, RS-122-2402, and RS-122-2403 has changed. In the new configuration, the library preparation reagents and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.
RNA-Seqテクノロジーにより均一なカバレッジが達成され、ストランド方向を正確に評価できます。さらに選択的転写物、アンチセンス発現、およびアリル特異的発現など、コーディングRNAとさまざまな形のノンコーディングRNAの両方にわたり高い信頼度で検出することができます。TruSeq Stranded Total RNA with Ribo-Zero Plant Kitでは、TruSeq RNA Library Preparation with Ribo-ZeroのリボソームRNA除去ケミストリーの利点を備えており、全トランスクリプトーム解析のための頑健で拡張性のある包括的ソリューションとなっています。
TruSeq Stranded Total RNA with Ribo-Zero Plant Kitでは、葉、種、および根の組織から細胞質、ミトコンドリア、および葉緑体のリボソームRNAを迅速かつ特異的に除去することができます。このキットは、シロイヌナズナ、イネ、およびトウモロコシなど複数の植物種での使用に関して検証されており、その他にも幅広い種で効率的なrRNA除去が実現します。*
*特定の生物種に関する情報については、イルミナテクニカルサポートにお問い合わせください。
| 装置 | 推奨サンプル数 | リード長 |
|---|---|---|
| NextSeq 550 System | RNA Profiling: 6-20 samples per run (based on 20 million reads per sample) | 2 x 75 bp |
| NextSeq 550 System | Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample) | 2 x 75 bp |
| HiSeq 2500 System | RNA Profiling: 30-200 samples per run (dual flow cell; based on 20 million reads per sample) | 2 x 75 bp |
| HiSeq 2500 System | Transcriptome Analysis: 12-80 samples per run (dual flow cell; based on 50 million reads per sample) | 2 x 75 bp |
| NovaSeq 6000 System | RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads. | ≤ 2 × 100 bp |
| NovaSeq 6000 System | Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads. | ≤ 2 × 100 bp |
Data Sheet | PDF | 5 versions
Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
Application Note | HTML
Illumina DNA PCR-Free Prep, Tagmentation
Data Sheet | PDF < 1 MB
TruSeq Stranded Total RNA Reference Guide Documentation
TruSeq Stranded Total RNA Checklist Documentation
TruSeq Stranded Total RNA Documentation
TruSeq Stranded Total RNA Consumables & Equipment Documentation
TruSeq Stranded Total RNA With Illumina Ribo-Zero Plus rRNA Depletion Reference Guide Documentation
カスタムプロトコルセレクタ
Generates customized, end-to-end instructions
単一ステップでrRNAおよびグロビンmRNAを除去する効率的なワークフローで、血液由来のRNAから全トランスクリプトームシーケンスライブラリーを調製します。
A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.
Limited Use Label License: The TruSeq Stranded Total RNA with Ribo-Zero Plant Workflow product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERCIAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.
