Processing more samples in less time

Sample multiplexing exponentially increases the number of samples sequenced per run

Multiplex Sequencing

Multiplex sequencing allows large numbers of libraries to be pooled and sequenced simultaneously during a single run on a high-throughput instrument. Sample multiplexing is useful for many applications, from targeted panels to whole human genome sequencing.

Individual "barcode" sequences are added to each DNA fragment during next-generation sequencing (NGS) library preparation so that each read can be identified and sorted before the final data analysis. Pooling samples exponentially increases the number of samples analyzed in a single run, without drastically increasing cost or time.

Conceptual Overview of Sample Multiplexing
  • Fast High-Throughput Strategy: Large sample numbers can be simultaneously sequenced during a single experiment
  • Cost-Effective Method: Sample pooling improves productivity by reducing time and reagent use
  • High-Quality Data: Accurate maintenance of read length of unknown sequences
  • Simplified Analysis: Automatic sample identification with "barcodes" using Illumina data analysis software
Multiplexing Facilitates Microbial Studies

Researchers leverage the speed and multiplexing capabilities of Illumina sequencers to identify microbes along the Mississippi River.

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Understanding UD Indexing

Learn how this approach can help ensure that your libraries will be sequenced and demultiplexed with the highest accuracy on Illumina sequencing platforms.

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Index Adapters Pooling Guide

This guide provides recommendations for preparing libraries with balanced index combinations for multiplexing and sequencing on Illumina systems.

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Pooling Calculator

This tool helps you calculate volumes and concentrations when pooling multiple libraries.

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Flexible High-Throughput Sequencer

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