Multiplex sequencing allows large numbers of libraries to be pooled and sequenced simultaneously during a single run on a high-throughput instrument. Sample multiplexing is useful for many applications, from targeted panels to whole human genome sequencing.
Individual "barcode" sequences are added to each DNA fragment during next-generation sequencing (NGS) library preparation so that each read can be identified and sorted before the final data analysis. Pooling samples exponentially increases the number of samples analyzed in a single run, without drastically increasing cost or time.
Two index options are available for multiplex sequencing: combinatorial dual (CD) indexes and unique dual (UD) indexes. In contrast to CD indexing, UD indexing has completely unique indexes.
Learn how this approach can help ensure that your libraries will be sequenced and demultiplexed with the highest accuracy on Illumina sequencing platforms.Read Bulletin
This guide provides recommendations for preparing libraries with balanced index combinations for multiplexing and sequencing on Illumina systems.Access Guide
This tool helps you calculate volumes and concentrations when pooling multiple libraries.Access Tool