TruSight Cardio Sequencing Kit for NextSeq v2 (FC-141-1011) has been discontinued; the recommended replacement is TruSight Cardio Sequencing Kit for NextSeq v2.5 (20035190). The library prep components of the kit have not changed. Please contact your local sales representative for additional information on this change.
TruSight Cardio Sequencing Kit for MiSeq and MiSeqDx (12 indexes, 12 samples, 1 enrichment)
FC-141-1010
Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents - 96 samples (8, 12-plex enrichment reactions)
20025524
The TruSight Cardio Kit uses next-generation sequencing (NGS) to provide comprehensive coverage of 174 genes with known associations to 17 ICCs, including cardiomyopathies, arrhythmias, aortopathies, and more. Genes were expertly selected with researchers at the National Heart Centre Singapore and Imperial College of London. Content includes genes known to be associated with hereditary heart disease and emerging genes found in the literature.1
Download this reference guide to understand which kit quantities you should purchase to support your research.
Download Reference Guide装置 | 推奨サンプル数 | リード長 |
---|---|---|
MiSeq System | 12 samples per run with v2 reagents (based on 300x mean coverage of targeted content) | 2 × 150 bp (max recommended) |
NextSeq 550 System | 48 samples per run (mid output; based on 300x mean coverage of targeted content) | 2 × 150 bp (max recommended) |
TruSight Cardio Sequencing Kit | TruSight Oneシーケンスパネル | |
---|---|---|
Content Specifications | 575 Kb genomic content (174 genes) | TruSight One: ~12 Mb genomic content (~4800 genes). TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes). |
Description | Targeted sequencing research panel to identify somatic variants associated with 17 inherited cardiac conditions. | Fixed research panels targeting exonic regions that harbor disease-causing variants. |
Input Quantity | 50 ng DNA | 50 ng DNA |
Method | Target Enrichment, Target Enrichment, Targeted DNA Sequencing | Target Enrichment, Target Enrichment, Targeted DNA Sequencing |
Multiplexing | Up to 96-plex | Up to 96-plex |
Specialized Sample Types | Not FFPE-Compatible | Not FFPE-Compatible |
Species Category | Human | Human |
TruSight Cardio Sequencing Kit Gene List
product_file | EXCEL < 1 MB
TruSight Fixed Panel Combo Order Quantities
Product Information Sheet | PDF < 1 MB
TruSight Cardio Sequencing Panel
Data Sheet | PDF < 1 MB
TruSight Cardio Sequencing Kit Checklist Documentation
TruSight Cardio Sequencing Kit Support Documentation
TruSight Cardio Sequencing Kit Reference Guide Documentation
カスタムプロトコルセレクタ
Generates customized, end-to-end instructions
The TruSight Cardiomyopathy Sequencing Panel has been discontinued. The TruSight Cardio Sequencing Kit is the recommended replacement.
* Data on file. Illumina, Inc. 2015.