TruSight Cardio Sequencing Kit

This kit provides researchers with a comprehensive, cost-effective solution for identifying causal variants implicated in inherited cardiac conditions (ICCs). Read More...
製品の選択
Bundled Solutions

TruSight Cardio Sequencing Kit for NextSeq v2.5 (48 indexes, 48 samples, 4 enrichments)

20035190

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TruSight Cardio Sequencing Kit for MiSeq and MiSeqDx (12 indexes, 12 samples, 1 enrichment)

FC-141-1010

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Enrichment Panels

TruSight Cardio – Enrichment Oligos only (8 Enrichment Reactions)

20029229

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アクセサリー製品

Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents - 96 samples (8, 12-plex enrichment reactions)

20025524

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Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents - 16 samples (16, 1-plex enrichment reactions)

20025523

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Nextera DNA Flex Pre-Enrichment Library Prep Reagents (96 samples)

20025520

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Nextera DNA Flex Pre-Enrichment Library Prep Reagents (16 samples)

20025519

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IDT® for Illumina Nextera DNA Unique Dual Indexes Set A (96 Indexes, 96 Samples)

20027213

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製品のハイライト

  • Expertly defined genes selected in collaboration with the Imperial College of London
  • 99% of the targeted regions covered at a depth of at least 20×*
  • Cost to sequence is ~ $1 US per gene; fully supported on Illumina sequencing and informatics platforms

The TruSight Cardio Kit uses next-generation sequencing (NGS) to provide comprehensive coverage of 174 genes with known associations to 17 ICCs, including cardiomyopathies, arrhythmias, aortopathies, and more. Genes were expertly selected with researchers at the National Heart Centre Singapore and Imperial College of London. Content includes genes known to be associated with hereditary heart disease and emerging genes found in the literature.1

Frequently Purchased Together

Plan on Using the TruSight Cardio Sequencing Kit with MiSeq v3?

Download this reference guide to understand which kit quantities you should purchase to support your research.

Download Reference Guide
Reference guide: TruSight Cardio Sequencing Kit

仕様

推奨プロジェクト

装置 推奨サンプル数 リード長
MiSeq System 12 samples per run with v2 reagents (based on 300x mean coverage of targeted content) 2 × 150 bp (max recommended)
NextSeq 550 System 48 samples per run (mid output; based on 300x mean coverage of targeted content) 2 × 150 bp (max recommended)

製品比較

TruSight Cardio Sequencing Kit TruSight Oneシーケンスパネル
Content Specifications 575 Kb genomic content (174 genes) TruSight One: ~12 Mb genomic content (~4800 genes).
TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes).
Description Targeted sequencing research panel to identify somatic variants associated with 17 inherited cardiac conditions. Fixed research panels targeting exonic regions that harbor disease-causing variants.
Input Quantity 50 ng DNA 50 ng DNA
Method Target Enrichment, Target Enrichment, Targeted DNA Sequencing Target Enrichment, Target Enrichment, Targeted DNA Sequencing
Multiplexing Up to 96-plex Up to 96-plex
Specialized Sample Types Not FFPE-Compatible Not FFPE-Compatible
Species Category Human Human

Method-Specific Workflow Example

 

The TruSight Cardiomyopathy Sequencing Panel has been discontinued. The TruSight Cardio Sequencing Kit is the recommended replacement.

Related Products to Consider

TruSight Oneシーケンスパネル

優れた分析感度かつ特異度で、エクソームの疾患関連領域をターゲットとする包括的なシーケンス研究パネルです。


Illumina DNA Prep with Enrichment

カスタムパネル、固定パネル、全エクソーム濃縮に適したターゲットリシーケンスを実現する幅広いアプリケーションに対応した迅速な統合ワークフロー


MiSeq Reagent Kits v2

MiSeq sequencing reagents in pre-filled, ready-to-use cartridges. Micro and nano formats are available for low output applications.


References
  1. Pua CJ, Bhalshankar J, Miao K, et al. Development of a comprehensive sequencing assay for inherited cardiac condition genes. J Cardiovasc Trans Res. 2016;9:3.

* Data on file. Illumina, Inc. 2015.