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TruSight Cardio Sequencing Kit for NextSeq v2 (FC-141-1011) has been discontinued; the recommended replacement is TruSight Cardio Sequencing Kit for NextSeq v2.5 (20035190). The library prep components of the kit have not changed. Please contact your local sales representative for additional information on this change.

TruSight Cardio Sequencing Kit

This kit provides researchers with a comprehensive, cost-effective solution for identifying causal variants implicated in inherited cardiac conditions (ICCs).Read More...

製品の選択

価格

Bundled Solutions

TruSight Cardio Sequencing Kit for NextSeq v2.5 (48 indexes, 48 samples, 4 enrichments)

20035190

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TruSight Cardio Sequencing Kit for MiSeq and MiSeqDx (12 indexes, 12 samples, 1 enrichment)

FC-141-1010

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Enrichment Panels

TruSight Cardio – Enrichment Oligos only (8 Enrichment Reactions)

20029229

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アクセサリー製品

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Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents - 96 samples (8, 12-plex enrichment reactions)

20025524

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Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents - 16 samples (16, 1-plex enrichment reactions)

20025523

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Nextera DNA Flex Pre-Enrichment Library Prep Reagents (96 samples)

20025520

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Nextera DNA Flex Pre-Enrichment Library Prep Reagents (16 samples)

20025519

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IDT® for Illumina Nextera DNA Unique Dual Indexes Set A (96 Indexes, 96 Samples)

20027213

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カートに追加

合計：

製品のハイライト

Expertly defined genes selected in collaboration with the Imperial College of London
99% of the targeted regions covered at a depth of at least 20×*
Cost to sequence is ~ $1 US per gene; fully supported on Illumina sequencing and informatics platforms

The TruSight Cardio Kit uses next-generation sequencing (NGS) to provide comprehensive coverage of 174 genes with known associations to 17 ICCs, including cardiomyopathies, arrhythmias, aortopathies, and more. Genes were expertly selected with researchers at the National Heart Centre Singapore and Imperial College of London. Content includes genes known to be associated with hereditary heart disease and emerging genes found in the literature.¹

Frequently Purchased Together

Plan on Using the TruSight Cardio Sequencing Kit with MiSeq v3?

Download this reference guide to understand which kit quantities you should purchase to support your research.

Download Reference Guide

Reference guide: TruSight Cardio Sequencing Kit

仕様

推奨プロジェクト

装置	推奨サンプル数	リード長
MiSeq System	12 samples per run with v2 reagents (based on 300x mean coverage of targeted content)	2 × 150 bp (max recommended)
NextSeq 550 System	48 samples per run (mid output; based on 300x mean coverage of targeted content)	2 × 150 bp (max recommended)

製品比較

	TruSight Cardio Sequencing Kit	TruSight Oneシーケンスパネル	TruSight Inherited Disease
Content Specifications	575 Kb genomic content (174 genes)	TruSight One: ~12 Mb genomic content (~4800 genes). TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes).	2.25 Mb genomic content (552 genes)
Description	Targeted sequencing research panel to identify somatic variants associated with 17 inherited cardiac conditions.	Fixed research panels targeting exonic regions that harbor disease-causing variants.	Fixed research panel focused on severe pediatric onset disorders.
Input Quantity	50 ng DNA	50 ng DNA	50 ng DNA
Method	Target Enrichment , Target Enrichment, Targeted DNA Sequencing	Target Enrichment , Target Enrichment, Targeted DNA Sequencing	Target Enrichment , Target Enrichment, Targeted DNA Sequencing
Multiplexing	Up to 96-plex	Up to 96-plex	Up to 96-plex
Specialized Sample Types	Not FFPE-Compatible	Not FFPE-Compatible	Not FFPE-Compatible
Species Category	Human	Human	Human

Prepare Library for:

Targeted Gene Sequencing

Sequence Efficiently with:

MiSeq System

NextSeq 550 System

Analyze Your Data with:

BaseSpace BWA Enrichment App

VariantStudio Software

製品に関する文献

Illumina VariantStudio Data Analysis Software

Data Sheet | PDF2 MB

TruSight Cardio Sequencing Kit

Data Sheet | PDF< 1 MB

TruSight Cardio Sequencing Kit Gene List

product_file | EXCEL< 1 MB

マニュアルとサポート情報

TruSight Cardio Sequencing Kit Checklist Documentation

TruSight Cardio Sequencing Kit Reference Guide Documentation

TruSight Cardio Sequencing Kit Support Documentation

カスタムプロトコルセレクタ
Generates customized, end-to-end instructions

すべて TruSight Cardio Sequencing Kit Support

TruSight Oneシーケンスパネル

Comprehensive sequencing research panels targeting disease-associated regions of the exome with high analytical sensitivity and specificity.

Nextera Flex for Enrichment

カスタムパネル、固定パネル、全エクソーム濃縮に適したターゲットリシーケンスを実現する幅広いアプリケーションに対応した迅速な統合ワークフロー

MiSeq Reagent Kits v2

MiSeq sequencing reagents in pre-filled, ready-to-use cartridges. Micro and nano formats are available for low output applications.

TruSight Inherited Disease

TruSight Inherited Disease is a targeted sequencing research panel that focuses on 552 genes associated with severe, recessive pediatric onset diseases.

References

Pua CJ, Bhalshankar J, Miao K, et al. Development of a comprehensive sequencing assay for inherited cardiac condition genes. J Cardiovasc Trans Res. 2016;9:3.

* Data on file. Illumina, Inc. 2015.