TruSight Oneシーケンスパネル
Comprehensive sequencing research panels targeting disease-associated regions of the exome with high analytical sensitivity and specificity.Read More...
TruSight Oneシーケンスパネルを使用すると、コストを管理しながら、ラボのシーケンスポートフォリオを拡大し効率化することができます。
このパネルは、臨床研究ラボにとって多様なアッセイポートフォリオを管理するための経済的なソリューションです。 TruSight One Sequencingパネルは>4800の疾患関連遺伝子をカバーしているのに対し、TruSight One Expanded Sequencingパネルは最近の文献で取り上げられた疾患関連1900遺伝子を追加しています。パネルに含まれているすべての遺伝子を解析することも、特定のサブセットに注目して解析することもできます。1回のアッセイで既存のメニューが拡大され、ワークフローの効率化またはシーケンスオプションの全ポートフォリオの作成が可能になります。
Download this reference guide to understand which kit quantities you should purchase to support your research.
Download Reference Guide
| 装置 | 推奨サンプル数 | リード長 |
|---|---|---|
| MiSeq System | TruSight One: 3 samples per run with v3 reagents (based on 100x mean coverage of targeted content) | Up to 2 × 150 bp |
| NextSeq 550 System | TruSight One (samples per run): mid output: 12, high output: 36 (based
on 100x mean coverage of targeted content) TruSight One Expanded: 24 samples per run (high output; based on 100x mean coverage of targeted content) |
Up to 2 × 150 bp Up to 2 × 150 bp |
| HiSeq 2500 System | TruSight One Expanded (samples per run): rapid run: 18, high output: 95 (based on 100x mean coverage of targeted content) | Up to 2 × 150 bp |
| NovaSeq 6000 System | TruSight One Expanded: 96 samples per run (S1 flow cell; based on 100x mean coverage of targeted content) | Up to 2 × 150 bp |
| TruSight Oneシーケンスパネル | TruSight Inherited Disease | TruSight Cardio Sequencing Kit | |
|---|---|---|---|
| Content Specifications | TruSight One: ~12 Mb genomic content (~4800 genes). TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes). |
2.25 Mb genomic content (552 genes) | 575 Kb genomic content (174 genes) |
| Description | Fixed research panels targeting exonic regions that harbor disease-causing variants. | Fixed research panel focused on severe pediatric onset disorders. | Targeted sequencing research panel to identify somatic variants associated with 17 inherited cardiac conditions. |
| Input Quantity | 50 ng DNA | 50 ng DNA | 50 ng DNA |
| Method | Target Enrichment , Target Enrichment, Targeted DNA Sequencing | Target Enrichment , Target Enrichment, Targeted DNA Sequencing | Target Enrichment , Target Enrichment, Targeted DNA Sequencing |
| Multiplexing | Up to 96-plex | Up to 96-plex | Up to 96-plex |
| Specialized Sample Types | Not FFPE-Compatible | Not FFPE-Compatible | Not FFPE-Compatible |
| Species Category | Human | Human | Human |
TruSight One Expandedパネルのサイズが大きいため、最適なシーケンスのカバレッジを実現するNextSeqシステム、HiSeqシステム、またはNovaSeqシリーズのシステムの使用をお勧めします。
Sequencing Panel for 4813 Genes with Known Associated Clinical Phenotypes
Technical Note | PDF< 1 MB
marker_list_-csv,_zip- | ZIP< 1 MB
TruSight One Expanded Panel Gene List
marker_list_-csv,_zip- | EXCEL< 1 MB
Data Sheet | PDF | 5 versions
Data Sheet | PDF1 MB
イルミナ VariantStudio データ解析ソフトウェア
Data Sheet | PDF1 MB
TruSight One Sequencing Panel Kits Support Documentation
カスタムプロトコルセレクタ
Generates customized, end-to-end instructions
For the 36-sample TruSight One Sequencing Panel (Catalog No. FC-141-1007), the instrument-specific sequencing reagents must be purchased separately.
This kit provides researchers with a comprehensive, cost-effective solution for identifying causal variants implicated in inherited cardiac conditions (ICCs).
BaseSpace Variant Interpreter allows scientists to rapidly identify biologically significant variants from human genomic data.
TruSight Inherited Disease is a targeted sequencing research panel that focuses on 552 genes associated with severe, recessive pediatric onset diseases.
Optimized chemistry to increase cluster density and read length, and improve sequencing quality scores, compared to earlier MiSeq reagent kit versions.
TruSight Rapid Capture all-in-one kits enable targeted sequencing library preparation and enrichment for up to 96 samples in only 1.5 days.
システムの主な消耗品は、どのシステムを使用するかに応じて、36サンプルのTruSight Oneシーケンシングパネル（カタログ番号FC-141-1007）用に別途購入します。MiSeqシステム 、NextSeqシステム 、またはHiSeqシリーズのシステム