NET-Seq

NET-Seq

Native elongating transcript sequencing (NET-Seq) maps transcription through the capture of 3’ RNA . In this method the RNA polymerase II elongation complex is immunoprecipitated, and RNA is extracted and reverse-transcribed to cDNA. Deep sequencing of the cDNA allows for 3’-end sequencing of nascent RNA, providing nucleotide resolution at transcription.

  • NET-Seq: Churchman L. S. and Weissman J. S. (2011) Nascent transcript sequencing visualizes transcription at nucleotide resolution. Nature 469: 368-373