TruSeq RNA Library Prep Kit v2

These kits provide a simple, cost-effective solution for analysis of the coding transcriptome, with minimal hands-on time. Read More...

TruSeq RNA Library Preparation Kit v2, Set A (48 samples, 12 indexes)



TruSeq RNA Library Preparation Kit v2, Set B (48 samples, 12 indexes)



Product Highlights

Generate mRNA-focused sequencing libraries from total RNA, with enhanced multiplex capability and a simple workflow with master-mixed reagents.

Multiplex Capabilities

Kits feature 24 unique indexes, delivering enhanced multiplex performance for processing large numbers of samples. The kits include adapters containing unique index sequences that are ligated to sample fragments at the beginning of the library construction process. This allows the samples to be pooled and then individually identified during downstream analysis.

Minimal Hands-On Steps

Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput RNA sequencing studies achieved with a user-friendly workflow.

Automation Options

Find an up-to-date list of automation vendors with robotic systems compatible with this kit

Frequently Purchased Together



Method-Specific Workflow Example


Customer Stories

Supporting Data and Figures

TruSeq RNA Library Preparation Reagents Provide Significant Savings in Time and Effort
TruSeq RNA Library Prep Reagents number of steps

Compared to current methods for preparing mRNA samples for sequencing, use of the TruSeq reagents significantly reduces the number of steps and hands-on time.




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TruSeq RNA Exome

Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.