TruSeq RNA Library Prep Kit v2
These kits provide a simple, cost-effective solution for analysis of the coding transcriptome, with minimal hands-on time.Read More...
Generate mRNA-focused sequencing libraries from total RNA, with enhanced multiplex capability and a simple workflow with master-mixed reagents.
Kits feature 24 unique indexes, delivering enhanced multiplex performance for processing large numbers of samples. The kits include adapters containing unique index sequences that are ligated to sample fragments at the beginning of the library construction process. This allows the samples to be pooled and then individually identified during downstream analysis.
Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput RNA sequencing studies achieved with a user-friendly workflow.
Find an up-to-date list of automation vendors with robotic systems compatible with this kit
| 装置 | 推奨サンプル数 | リード長 |
|---|---|---|
| NextSeq 550 System | RNA Profiling: 13–40 samples per run (based on 10 million reads per
sample) Transcriptome Analysis: 5–16 samples per run (based on 25 million reads per sample) |
2 x 75 bp |
| HiSeq 2500 System | RNA Profiling: 60–400 samples per run (dual flow cell; based on 10
million reads per sample) Transcriptome Analysis: 24–160 samples per run (dual flow cell; based on 25 million reads per sample) |
2 x 75 bp |
Compared to current methods for preparing mRNA samples for sequencing, use of the TruSeq reagents significantly reduces the number of steps and hands-on time.
TruSeq RNA and DNA Library Preparation Kits v2
Data Sheet | PDF< 1 MB
TruSeq RNA Library Prep Kit v2 Documentation
Illumina Experiment Manager User Guide Documentation
Sequencing Library qPCR Quantification Guide Documentation
TruSeq Sample Prep Best Practices Guide Documentation
TruSeq RNA Sample Prep v2 Guide Documentation
カスタムプロトコルセレクタ
Generates customized, end-to-end instructions
Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.
A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.
