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RNA Exome Capture Sequencing

Introduction to RNA Exome Capture Sequencing

Achieve cost-effective, accurate, and sensitive RNA exome analysis of even difficult samples without sacrificing gene fusion discovery power. Many RNA exome sequencing methods focus on a defined number of known transcripts or require expensive deep sequencing. RNA exome capture sequencing overcomes these challenges by combining RNA-Seq with exome enrichment.

This method captures only the coding regions of the transcriptome, allowing higher throughput and requiring lower sequencing depth than non-exome capture methods. Sequence-specific capture of the RNA exome does not rely on the presence of a poly-A tail. This makes RNA exome capture sequencing ideal for RNA-Seq with low-quality samples or limited starting material.

Advantages of RNA Exome Capture Sequencing

Isolating transcriptome coding regions maximizes discovery power at a fraction of the read depth of total RNA sequencing.

  • Enables high sample throughput and cost efficiency
  • Focuses on high-value content for affordability
  • Achieves high-quality data from degraded samples, including formalin-fixed, paraffin-embedded (FFPE) tissues
  • Requires low sample input (as little as 10 ng total RNA) while maintaining high sensitivity
Transcriptome Analysis with NGS

Illumina Distinguished Scientist Dr. Gary Schroth demonstrates the latest advances in RNA-Seq technology. Learn how the latest RNA library prep methods allow transcriptome analysis from challenging samples, like single cells or FFPE tissues.

View Video

RNA Exome Capture Sequencing Workflow

Illumina sequencing by synthesis (SBS) chemistry is the most widely adopted NGS technology, generating more than 90% of global sequencing data.*

In addition to industry-leading data quality, Illumina offers integrated RNA exome capture workflows that simplify the entire process, from library preparation to data analysis and biological interpretation.

Featured Products

NextSeq 1000 & 2000 Systems
NextSeq 1000 & 2000 Systems

Groundbreaking benchtop sequencers allow you to explore new discoveries across a variety of current and emerging applications, with higher efficiency and fewer restraints.

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Illumina Ribo-Zero Plus rRNA Depletion Kit
Illumina Ribo-Zero Plus rRNA Depletion Kit

The stand-alone Illumina Ribo-Zero Plus kit allows for ribosomal RNA removal in human, mouse, rat, and bacterial samples.

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Illumina RNA Prep with Enrichment
Illumina RNA Prep with Enrichment

Achieve rapid, targeted interrogation of an expansive number of target genes with exceptional capture efficiency and coverage uniformity.

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RNA シーケンス検討事項

遺伝子発現プロファイリング、ターゲットRNA発現、small RNA解析、など、それぞれのRNA-Seq実験によって、必要とされるリード長や深度は異なります。このページでは、実験において考慮すべき点をレビューしています。実験計画にご活用ください。

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Additional Resources

User-Friendly RNA-Seq Data Analysis
User-Friendly RNA-Seq Data Analysis

Access user-friendly tools that support a broad range of gene expression and transcriptome analysis studies.

Buyer’s Guide: Simple RNA-Seq Workflows
Buyer’s Guide: Simple RNA-Seq Workflows

Evaluating options for next-generation RNA sequencing.

Gene Panel and Array Finder
Gene Panel and Array Finder

プロジェクトに適したシーケンスパネルまたはマイクロアレイを使用してさらに研究を進めてください

*Data calculations on file. Illumina, Inc., 2015