Small RNA Sequencing

Introduction to Small RNA Sequencing

Small noncoding RNAs act in gene silencing and post-transcriptional regulation of gene expression. Small RNA sequencing (RNA-Seq) is a technique to isolate and sequence small RNA species, such as microRNAs (miRNAs). Small RNA-Seq can query thousands of small RNA and miRNA sequences with unprecedented sensitivity and dynamic range.

With small RNA-Seq you can discover novel miRNAs and other small noncoding RNAs, and examine the differential expression of all small RNAs in any sample. You can characterize variations such as isomiRs with single-base resolution, as well as analyze any small RNA or miRNA without prior sequence or secondary structure information.

Advantages of Small RNA Sequencing

Generate miRNA sequencing libraries directly from total RNA to understand the role of noncoding RNA.

  • Understand how post-transcriptional regulation contributes to phenotype
  • Identify novel biomarkers
  • Capture the complete range of small RNA and miRNA species
Epigenetic Analysis and Small RNA-Seq

Illumina offers a broad portfolio of epigenetic analysis tools for investigators interested in studying epigenetic modifications and small RNA gene regulation.

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RNA シーケンス検討事項

遺伝子発現プロファイリング、ターゲットRNA発現、small RNA解析、など、それぞれのRNA-Seq実験によって、必要とされるリード長や深度は異なります。このページでは、実験において考慮すべき点をレビューしています。実験計画にご活用ください。

RNA シーケンス検討事項

Honeysuckle, miRNA, and The Flu

Find out how researchers used Illumina sequencing technology to study a honeysuckle-encoded microRNA that targets influenza A viruses.

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Launch Modal

Small RNA-Seq Workflow

Illumina sequencing by synthesis (SBS) chemistry is the most widely adopted NGS technology, generating approximately 90% of sequencing data worldwide.*

In addition to our industry-leading data quality, Illumina offers a simple workflow for small RNA and miRNA sequencing that simplifies the entire process, from library preparation to data analysis and biological interpretation.

Access Illumina Quality NGS with the MiniSeq Benchtop Sequencer

Perform small RNA-Seq with a sequencing solution that fits your benchtop, your budget, and your workflow. Discover novel miRNAs and analyze any small noncoding RNA without prior sequence or secondary structure information.

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Related Solutions

Cancer Research: Variant Detection

Small RNA sequencing enables the discovery profiling of miRNAs and other small noncoding RNAs present in the cancer transcriptome at extremely high coverage. Learn more about cancer transcriptome studies.

Complex Disease: RNA Editing

NGS-based profiling enables rapid, high-throughput analysis of the miRNA transcriptome, and can provide insights into complex disease-related RNA editing processes and mutational events. Learn more about complex disease research.

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Additional Resources

Small RNAシーケンスのベストプラクティスに関するウェビナー
Small RNAシーケンスのベストプラクティスに関するウェビナー

Small RNAライブラリー調製、シーケンス、および基礎データ解析の考慮事項。



TruSeq Small RNA Library Kit の FAQ
TruSeq Small RNA Library Kit の FAQ

Small RNA-Seq についてよくある質問に対する回答をご覧ください。

RNA-Seq と NextSeq シリーズ
RNA-Seq と NextSeq シリーズ


*Data calculations on file. Illumina, Inc., 2015