Respiratory Virus Oligo Panel

Highly sensitive detection and characterization of common respiratory viruses, including COVID-19 strains, with comprehensive, rapid target enrichment sequencing. Read More...
製品の選択
Which products do I need?
Panel

Respiratory Virus Oligo Panel v2

20044311

価格
 
 

Library Prep

Illumina RNA Prep with Enrichment (L) Tagmentation 16 Samples

20040536

価格
 
 

Illumina RNA Prep with Enrichment (L) Tagmentation 96 Samples

20040537

価格
 
 

Index Adapters

IDT for Illumina - DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

価格
 
 

IDT for Illumina - DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20027214

価格
 
 

製品のハイライト

Using the Respiratory Virus Oligo Panel with Illumina RNA Prep with Enrichment allows researchers to obtain next-generation sequencing (NGS) data that can confirm the presence of SARS-CoV-2 and advance variant analysis studies. The agnostic design allows for widespread identification of pathogenic respiratory viruses.

Target Enrichment for Respiratory Virus Detection

Target enrichment through hybrid–capture methods allows for highly sensitive detection, without requiring the high read depth needed for shotgun metagenomic sequencing. Additionally, this method allows for near-complete sequence data of targets and opens up applications such as variant analysis for viral evolution or viral surveillance.1

Compared to other targeted resequencing methods, such as amplicon sequencing, enrichment through hybrid capture allows for dramatically larger probe panels with more comprehensive profiling of the target regions. Additionally, the oligo probes used for hybrid–capture protocols remain effective even within highly mutagenic regions, allowing targeting of rapidly evolving viruses, such as RNA viruses.

Streamlined Workflow

This comprehensive workflow integrates sample preparation, library preparation, target enrichment, sequencing, and data analysis. The workflow is intended to enrich viral targets from total nucleic acid extraction.

Reverse transcription of extracted RNA and library preparation is performed using Illumina RNA Prep with Enrichment, followed by enrichment with the Illumina Respiratory Virus Oligo Panel. After sequencing on an Illumina NGS system, data analysis proceeds using the DRAGEN Pathogen Detection pipeline and IDbyDNA Explify Platform.

  1. Gaudin M and Desnues C. Hybrid Capture-Based Next Generation Sequencing and Its Application to Human Infectious Diseases. Front Microbiol. 2018; 9:2924.

Frequently Purchased Together

仕様

製品比較

Respiratory Virus Oligo Panel Respiratory Pathogen ID/AMR Enrichment Panel Kit (Powered by IDbyDNA Explify)
Automation Capability Liquid Handling Robots Liquid Handling Robots
Species Category Human, Virus Bacteria, Virus
System Compatibility MiniSeq , MiSeq , NextSeq 550 MiniSeq , MiSeq , NextSeq 550

Method-Specific Workflow Example

 

Supporting Data and Figures

 

Related Products