AmpliSeq for Illumina BRCAパネルは、 BRCA1およびBRCA2遺伝子の体細胞および生殖細胞系列変異体の研究のためのターゲットリシーケンスアッセイです。主な特長は次のとおりです。
BRCAパネルは、AmpliSeq for Illuminaのポリメラーゼ連鎖反応（PCR）ベースのライブラリー調製、イルミナの1塩基合成（SBS）次世代シーケンス（NGS）技術、自動化解析を含む統合ワークフローの一部です。
BRCA1およびBRCA2は、特定の突然変異を有する場合、乳がんおよび卵巣がんのリスク増加に関与するがん抑制遺伝子です。このカタログパネルは、ターゲットを特定し、プライマーを設計し、パネルを最適化する時間と労力を節約します。
| 装置 | 推奨サンプル数 | リード長 |
|---|---|---|
| iSeq 100 System | 12 samples per run (assumes minimum coverage of 500×) 96 samples per run (assumes minimum coverage of 50x) |
2 × 150 bp 2 × 150 bp |
| MiniSeq System | Samples per run: mid output: 24, high output: 80 (assumes minimum coverage of 500×) | 2 × 150 bp |
| MiSeq System | Samples per run at 500x minimum coverage (by reagent kit version): v2 nano: 3, v2 micro: 12, v2: 48, v3: 80 Samples per run at 50x minimum coverage (by reagent kit version): v2 nano: 32, v2 micro: 96 |
2 × 150 bp 2 × 150 bp |
| AmpliSeq for Illumina BRCA Panel | TruSight Cancer | |
|---|---|---|
| Assay Time | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 1.5 days |
| Cancer Type | Solid Tumor | Pan-Cancer |
| Content Specifications | Exonic regions and the flanking intronic sequences of the BRCA1 and BRCA2 | Fixed set of probes that enrich for 94 genes and 284 SNPs associated with common and rare cancers. |
| Description | Germline and somatic analysis studies of BRCA1 and BRCA2. | Germline mutation detection research for common and rare cancers. |
| Hands-On Time | < 1.5 hours | 5 hours |
| Input Quantity | 1–100 ng (10 ng recommended per pool) | 50 ng DNA |
| Method | Amplicon Sequencing, Targeted DNA Sequencing | Target Enrichment, Target Enrichment, Targeted DNA Sequencing |
| Multiplexing | 96 dual index combinations | Up to 96-plex |
| Specialized Sample Types | Blood, FFPE Tissue | Not FFPE-Compatible |
| Species Category | Human | Human |
| Variant Class | Germline Variants, Insertions-Deletions (indels), Single Nucleotide Polymorphisms (SNPs), Somatic Variants | Germline Variants |
AmpliSeq for Illumina Oncology Panels on the iSeq 100 System
Application Note | PDF < 1 MB
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
AmpliSeq for Illumina BRCA Panel Data Sheet
Data Sheet | HTML
Index Adapters Pooling Guide Documentation
AmpliSeq for Illumina Direct FFPE DNA Kit Reference Guide Documentation
AmpliSeq for Illumina BRCA Panel Checklist Documentation
AmpliSeq for Illumina BRCA Panel Consumables & Equipment Documentation
Illumina Adapter Sequences Documentation
AmpliSeq for Illumina Custom and Community Panels Documentation
AmpliSeq for Illumina BRCA Panel Reference Guide Documentation
AmpliSeq for Illumina BRCA Panel Support - Documentation & Literature
カスタムプロトコルセレクタ
Generates customized, end-to-end instructions
These sequencing reagent kits offer increased stability and robustness over the v2 kit version.
MiSeq sequencing reagents in pre-filled, ready-to-use cartridges. Micro and nano formats are available for low output applications.
iSeq 100 Reagents feature a ready-to-use cartridge and one-channel SBS technology for simple, low-cost sequencing.
