Multiomic Sequencing Methods

Multiomics methods

Explore a wide array of sequencing methods for omics applications

Simplifying the approach to multiomics

Understanding which multiomics approach to use in your next experiment can be a daunting process. Our table helps you navigate and select the various omics studies with solutions that best suit your needs. Explore benefits and solutions for multiomics applications such as bulk sequencing, single-cell, and spatial sequencing methods.

Bulk sequencing

Bulk sequencing refers to an approach that pools cell populations, tissues, and other samples to arrive an average measurement. This method is useful for a wider breadth of analysis that is also cost effective when evaluating differences between samples.

  Key Benefit Library Prep System(s) Informatics

Transcriptomics + Proteomics

BEN-Seq Get gene expression and surface protein expression profiles simultaneously
  Key Benefit Library Prep System(s) Informatics

Liquid Biopsy

ctDNA Sequencing Noninvasive approach detecting ctDNA in plasma from solid tumors
  Key Benefit Library Prep System(s) Informatics

Solid Tumor

Targeted Cancer Screening Identify all mutational classes and IO biomarkers in a single assay

Single-cell sequencing

Single-cell sequencing is an advanced method that allows analysis of omic sequences at the resolution of individual cells. Such an approach allows researchers to comprehensively evaluate heterogeneity of populations that otherwise might be missed in bulk sequencing studies.

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RNA Single-Cell RNA-Seq Reveal transcriptome cell-to-cell variation Single-Cell RNA-Seq method is compatible with the NovaSeq 6000 and NextSeq 2000 platforms, with library prep and data analysis provided by 10X Genomics or through the Illumina DRAGEN Single Cell BaseSpace App. Learn More at 10X
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Epigenetics Single-Cell ATAC-Seq Access chomatin accessibility patterns to view cell-to-cell variation Single-Cell ATAC-Seq method is compatible with the NovaSeq 6000 and NextSeq 2000 platforms, with library prep and data analysis provided by 10X Genomics and open source tools such as Bowtie 2 and Seurat by the Satija Lab. Learn More at 10X
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RNA + Epigenetics Single-Cell Gene Expression + ATAC-Seq Correlate transcriptome and chomatin accessibility patterns at the single-cell level Utilize the Multiome Single-Cell ATAC-Seq + gene expression prep kit from 10X genomic on the NovaSeq 6000 and NextSeq 2000 platforms. Download Tech Note
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RNA + Proteomics CITE-Seq Evaluate gene expression and surface protein expression levels simultaneously at single-cell resolution CITE-Seq is compatible with the NovaSeq 6000 and NextSeq 2000 platforms, with compatible antibodies from BD or BioLegend and single-cell prep kits from 10X Genomics. Analysis tools can also be found from 10x Genomics, BD or BioLegend. Learn more about BioLegend solutions
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DNA + Protein Proteomics Correlate known tumor genomic variants to cell-surface proteins at single-cell resolution The Mission Bio DNA + protein panel for tumor samples is compatible with the NextSeq 2000 platform, with compatible antibodies from BioLegend and the prep instrument, prep kit, and analysis tools from Mission Bio Learn more about Mission Bio's Tapestri Platform

Spatial sequencing methods

Spatial sequencing methods offers a contextual view of cell activity and information as it combines omic information within intact tissues. This allows researchers to gain unprecedented insights to unravel the structure and function of complex tissues.

Spatial Methods
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RNA RNA-Seq Spatial Understand the gene expression patterns from a particular region of interest within FFPE or fresh frozen tissue sections RNA-Seq spatial sequencing is compatible with NovaSeq 6000 and NextSeq 2000 Platforms, with library prep and data analysis provided by NanoString Download Tech Note
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RNA + Proteomics CITE-Seq Spatial Correlate targeted gene expression and protein expressions patterns from FFPE or fresh frozen tissue sections CITE-Seq Spatial sequencing is compatible with NovaSeq 6000 and NextSeq 2000 Platforms, with library prep and data analysis provided by NanoString Learn more about Nanostring solutions