Illumina DNA Prep with Enrichment Training
20028457
Nextera Flex for Enrichmentは、イルミナのライブラリー調製用製品ラインの中でも最も迅速かつ柔軟なDNAターゲットシーケンスソリューションです：
Nextera Flex for Enrichmentでは、迅速かつ使いやすいワークフローが採用されています。ビーズ上でのタグメンテーションケミストリーと簡略化された一度のハイブリダイゼーションプロトコールを組み合わせ、ワークフロー全体にかかる時間を約6時間半まで短縮しました。DNAのインプットと抽出からライブラリーのノーマライゼーション、濃縮、および濃縮後増幅まで、わずか6時間半で完了します。合計のハンズオン時間は約2時間にまで短縮されます。
Nextera Flex for Enrichmentは、幅広いDNAインプット範囲（50～1000 ng）および複数のサンプルタイプ（血液、唾液、ゲノムDNA、ホルマリン固定包埋（FFPE）組織など）に対応しています。血液サンプルや唾液サンプルを直接Nextera DNA Flex tagmentation reactionに追加することで必要な定量ステップが減るため、時間と費用を節約できます。
Nextera Flex for Enrichmentソリューションは、さまざまな研究用途に対応しながら、幅広いDNAインプット量で一貫したインサートサイズ、均一なカバレッジ、そして最適化された性能を実現します。ビーズベースのテクノロジーでバイアスおよびエラーの可能性が最小限に抑えられるため、あらゆるイルミナシーケンスシステムにわたり再現性の高いデータが得られます。
Discover the power that Illumina library prep tagmentation technology can bring to your lab through this interactive experience. Our library prep solutions offer whole-genome sequencing across various applications, flexibility across Illumina sequencers, and integrated sample input for blood and saliva without quantification.
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| 装置 | 推奨サンプル数 | リード長 |
|---|---|---|
| iSeq 100 System | TruSight Cancer: 4 samples per run (based on 280x mean targeted coverage; 99% target coverage at 20x) TruSight Cardio: 4 samples per run (based on 170x mean targeted coverage; 100% target coverage at 20x) |
2 x 100 bp 2 x 100 bp |
| MiniSeq System | TruSight Cancer (samples per run): mid output: 8, high output: 25 (based on 280x mean targeted coverage; 99% target coverage at 20x) TruSight Cardio (samples per run): mid output: 8, high output: 25 (based on 170x mean targeted coverage; 100% target coverage at 20x) |
2 x 100 bp 2 x 100 bp |
| MiSeq System | TruSight Cancer (samples per run): v2 reagents: 15, v3 reagents: 25 (based on 280x mean targeted coverage; 99% target coverage at 20x) TruSight Cardio (samples per run): v2 reagents: 15, v3 reagents: 25 (based on 170x mean targeted coverage; 100% target coverage at 20x) TruSight One (samples per run): v2 reagents: 1, v3 reagents: 3 (based on 120x mean targeted coverage; 95% target coverage at 20x) |
2 x 100 bp 2 x 100 bp 2 x 100 bp (up to 2 x 150 bp is possible) |
| NextSeq 550 System | Exome (samples per run): mid output: 5, high output: 16 (based on 50x mean targeted coverage; 90% target coverage at 20X) TruSight One (samples per run): mid output: 12, high output: 36 (based on 120x mean targeted coverage; 95% target coverage at 20x) TruSight One Expanded (samples per run): mid output: 7, high output: 24 (based on 130x mean targeted coverage; 97% target coverage at 20x) |
2 x 100 bp 2 x 100 bp (up to 2 x 150 bp is possible) 2 x 100 bp (up to 2 x 150 bp is possible) |
| HiSeq 2500 System | Exome (samples per run, single flow cell): rapid run: 12, high output with v4 reagents: 80 (based on 50x mean targeted coverage; 90% target coverage at 20X) TruSight One (samples per run, single flow cell): rapid run: 27, high output with v4 reagents: 184 (based on 120x mean targeted coverage; 95% target coverage at 20x) TruSight One Expanded (samples per run, single flow cell): rapid run: 18, high output with v4 reagents: 121 (based on 130x mean targeted coverage; 97% target coverage at 20x) |
2 x 100 bp 2 x 100 bp (up to 2 x 150 bp is possible) 2 x 100 bp |
| HiSeq 4000 System | TruSight One Expanded (samples per run, single flow cell): 151 (based on 130x mean targeted coverage; 97% target coverage at 20x) | 2 x 100 bp |
| NovaSeq 6000 System | Exome (samples per run, by flow cell type): S1: 128, S2: 164, S4: 400 (based on 50x mean targeted coverage; 90% target coverage at 20X; S4 limited by available indexes) | 2 x 100 bp |
Tumor exome sequencing with Illumina DNA Prep with Enrichment and the DRAGEN Platform
Application Note | PDF 1 MB
Somatic Variant Detection in FFPE Samples with Nextera Flex for Enrichment
Application Note | HTML
Whole-Exome Sequencing with Nextera Flex for Enrichment
Application Note | HTML
Fast, Flexible, and Self-Normalizing Library Prep with Nextera Flex Chemistry
Application Note | HTML
Data Sheet | HTML | 7 versions
Illumina library preparation solutions
Brochure | PDF 2 MB
NextSeq 550 exome sequencing solution
Application Note | PDF < 1 MB
NextSeq 1000 and NextSeq 2000 exome sequencing solution
Application Note | PDF < 1 MB
User-definable parameters in the Illumina DNA Prep with Enrichment workflow
Technical Note | PDF < 1 MB
Data Sheet | PDF | 5 versions
Index Adapters Pooling Guide Documentation
Illumina DNA Prep with Enrichment Checklist Documentation
Enrichment BaseSpace App Documentation
Illumina DNA Prep with Enrichment Reference Guide Documentation
Illumina Adapter Sequences Documentation
Illumina DNA Prep with Enrichment Documentation
Illumina DNA Prep with Enrichment Consumables & Equipment Documentation
カスタムプロトコルセレクタ
Generates customized, end-to-end instructions
Targeted panel investigating the exonic regions of 409 genes with known associations to cancer.
These sequencing reagent kits offer increased stability and robustness over the v2 kit version.
次世代シーケンサーとエピジェネティクスの知見を組み合わせたこのキットは、バイオマーカーの発見を加速させ、遺伝子制御におけるメチル化の役割を理解するのに役立ちます。
少量のDNAインプットで、小さなゲノム、PCRアンプリコン、およびプラスミドのシーケンスライブラリーを90分以内で調製できます。
ケミストリーの最適化により、以前のMiSeq試薬キットバージョンと比較して、クラスター密度とリード長が増加し、シーケンスのクオリティスコアが改善されました。
*Results from example data set. Actual performance may vary.
