Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents - 96 Samples（8, 12-plex enrichment reactions）
20025524
Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents - 16 Samples（16, 1-plex enrichment reactions）
20025523
Illumina FFPE QC Kit（384 reactions）
WG-321-1001
Nextera Flex for Enrichmentは、イルミナのライブラリー調製用製品ラインの中でも最も迅速かつ柔軟なDNAターゲットシーケンスソリューションです：
Nextera Flex for Enrichmentでは、迅速かつ使いやすいワークフローが採用されています。ビーズ上でのタグメンテーションケミストリーと簡略化された一度のハイブリダイゼーションプロトコールを組み合わせ、ワークフロー全体にかかる時間を約6時間半まで短縮しました。DNAのインプットと抽出からライブラリーのノーマライゼーション、濃縮、および濃縮後増幅まで、わずか6時間半で完了します。合計のハンズオン時間は約2時間にまで短縮されます。
Nextera Flex for Enrichmentは、幅広いDNAインプット範囲（50～1000 ng）および複数のサンプルタイプ（血液、唾液、ゲノムDNA、ホルマリン固定包埋（FFPE）組織など）に対応しています。血液サンプルや唾液サンプルを直接Nextera DNA Flex tagmentation reactionに追加することで必要な定量ステップが減るため、時間と費用を節約できます。
Nextera Flex for Enrichmentソリューションは、さまざまな研究用途に対応しながら、幅広いDNAインプット量で一貫したインサートサイズ、均一なカバレッジ、そして最適化された性能を実現します。ビーズベースのテクノロジーでバイアスおよびエラーの可能性が最小限に抑えられるため、あらゆるイルミナシーケンスシステムにわたり再現性の高いデータが得られます。
Discover the power that Nextera technology can bring to your lab through this interactive experience. Nextera library prep kit solutions offer whole-genome sequencing across various applications, flexibility across Illumina sequencers, and integrated sample input for blood and saliva without quantification.
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| 装置 | 推奨サンプル数 | リード長 |
|---|---|---|
| iSeq 100 System | TruSight Cancer: 4 samples per run (based on 280x mean targeted
coverage; 99% target coverage at 20x) TruSight Cardio: 4 samples per run (based on 170x mean targeted coverage; 100% target coverage at 20x) |
2 x 100 bp 2 x 100 bp |
| MiniSeq System | TruSight Cancer (samples per run): mid output: 8, high output: 25 (based
on 280x mean targeted coverage; 99% target coverage at 20x) TruSight Cardio (samples per run): mid output: 8, high output: 25 (based on 170x mean targeted coverage; 100% target coverage at 20x) |
2 x 100 bp 2 x 100 bp |
| MiSeq System | TruSight Cancer (samples per run): v2 reagents: 15, v3 reagents: 25
(based on 280x mean targeted coverage; 99% target coverage at 20x) TruSight Cardio (samples per run): v2 reagents: 15, v3 reagents: 25 (based on 170x mean targeted coverage; 100% target coverage at 20x) TruSight One (samples per run): v2 reagents: 1, v3 reagents: 2 (based on 120x mean targeted coverage; 95% target coverage at 20x) |
2 x 100 bp 2 x 100 bp 2 x 100 bp (up to 2 x 150 bp is possible) |
| NextSeq 550 System | Exome (samples per run): mid output: 5, high output: 16 (based on 50x
mean targeted coverage; 90% target coverage at 20X) TruSight One (samples per run): mid output: 12, high output: 36 (based on 120x mean targeted coverage; 95% target coverage at 20x) TruSight One Expanded (samples per run): mid output: 7, high output: 24 (based on 130x mean targeted coverage; 97% target coverage at 20x) |
2 x 100 bp 2 x 100 bp (up to 2 x 150 bp is possible) 2 x 100 bp (up to 2 x 150 bp is possible) |
| HiSeq 2500 System | Exome (samples per run, single flow cell): rapid run: 12, high output
with v4 reagents: 80 (based on 50x mean targeted coverage; 90% target
coverage at 20X) TruSight One (samples per run, single flow cell): rapid run: 27, high output with v4 reagents: 184 (based on 120x mean targeted coverage; 95% target coverage at 20x) TruSight One Expanded (samples per run, single flow cell): rapid run: 18, high output with v4 reagents: 121 (based on 130x mean targeted coverage; 97% target coverage at 20x) |
2 x 100 bp 2 x 100 bp (up to 2 x 150 bp is possible) 2 x 100 bp |
| HiSeq 4000 System | TruSight One Expanded (samples per run, single flow cell): 151 (based on 130x mean targeted coverage; 97% target coverage at 20x) | 2 x 100 bp |
| NovaSeq 6000 System | Exome (samples per run, by flow cell type): S1: 128, S2: 164, S4: 400 (based on 50x mean targeted coverage; 90% target coverage at 20X; S4 limited by available indexes) | 2 x 100 bp |
Somatic Variant Detection in FFPE Samples with Nextera Flex for Enrichment
Application Note | HTML
Fast, Flexible, and Self-Normalizing Library Prep with Nextera Flex Chemistry
Application Note | HTML
Data Sheet | HTML | 1 versions
Nextera Flex for Enrichment Checklist Documentation
Nextera Flex for Enrichment Consumables & Equipment Documentation
Enrichment BaseSpace App Documentation
Nextera Flex for Enrichment Reference Guide Documentation
Illumina Adapter Sequences Document Documentation
Index Adapters Pooling Guide Documentation
カスタムプロトコルセレクタ
Generates customized, end-to-end instructions
ヒト全ゲノムシーケンスからアンプリコン、プラスミド、微生物ゲノムまで、幅広いアプリケーションに対応した短時間の完結したワークフロー。
