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The TruSight Oncology 500 High-Throughput assay is built on the foundation of the TruSight Oncology 500 assay, with flexibility for higher sample throughput and batching options.
Consolidate Multiple Biomarker Assays into One
Streamlined Sample-to-Results Workflow
High Sample Throughput
Achieve Highly Confident Results
Enable In-House Comprehensive Genomic Profiling
*Automation available on the Beckman Coulter i-Series or the Hamilton Microlab STAR. Contact Illumina Sales for more information.
All three TruSight Oncology 500 assays are designed to identify key biomarkers in guidelines and clinical trials.
Assess key cancer biomarkers from FFPE tumor tissue with the TruSight Oncology 500 Assay.
Batch up to 192 samples at a time while using the same biomarkers and tissue type as the TruSight Oncology 500 assay.
Analyze circulating tumor DNA from plasma with the TruSight Oncology 500 ctDNA assay.
TruSight Oncology 500 High-Throughput | TruSight Oncology 500 | TruSight Oncology 500 ctDNA | |
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Cancer Type | Pan-Cancer | Pan-Cancer | Pan-Cancer |
Content Specifications | Targeted selection of DNA from 523 genes of interest, and RNA from 55 genes, for a total of 1.94Mb panel size. • Immuno-oncology Biomarker Coverage: TMB and MSI • Guideline Coverage: Broad coverage of key guidelines for multiple solid tumor types • Clinical Trials Coverage: Over 600 clinical trials* |
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Hands-On Time | Manual: ~10.5 hrs Automated: ~2.5 hrs |
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Input Quantity | 40 ng DNA, 40-80 ng RNA (need at least 2 mm3 FFPE tissue) | ||
Sample Throughput | 16–192 samples per flow cell |
* Based on Pierian clinical knowledgebase, as of February 2023.
† NovaSeq 6000Dx System in RUO Mode requires a separate, stand-alone DRAGEN server for secondary analysis.
Simplifying the process to identify applicable variants in oncology samples
Brochure | PDF 2 MB
Optimizing RNA input to detect gene fusions with TruSight Oncology 500 High-Throughput
Technical Note | PDF < 1 MB
Data analysis and reporting for the TruSight Oncology 500 portfolio
Product Information Sheet | PDF 1 MB
Automating TruSight Oncology 500 High-Throughput
Technical Note | PDF < 1 MB
Data Sheet | PDF | 7 versions
TruSight Oncology 500 High Throughput Checklist Documentation
DRAGEN TruSight Oncology 500 Analysis Software v2.1 Documentation
DRAGEN TruSight Oncology 500 Analysis Software v1.1 Documentation
TruSight Oncology 500 Analysis Software Documentation
TruSight Oncology 500 v2.0 Local App Documentation
DRAGEN TruSight Oncology 500 Analysis Software v2.1.1 on ICA Documentation
TruSight Oncology 500 v2.1 Local App Documentation
TruSight Oncology 500 High Throughput Reference Guide Documentation
TruSight Oncology 500 v2.3 Local App Documentation
DRAGEN TruSight Oncology 500 Analysis Software v1.1 on ICA Documentation
TruSight Oncology 500 v1.0 Local App Documentation
TruSight Oncology 500 High Throughput Consumables & Equipment Documentation
DRAGEN TruSight Oncology 500 Analysis Software v2.1.2 on ICA Documentation
TruSight Oncology 500 v2.2 Local App Documentation
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