TruSeq Custom Amplicon is a fully customizable, amplicon-based assay for targeted resequencing. The assay enables you to focus interrogation on key regions of genomic interest. Up to 1536 amplicons can be sequenced in a single reaction using a simple workflow. This highly targeted approach enables researchers to efficiently discover, validate, and screen genetic variants.
|TruSeq Custom Amplicon v1.5 (discontinued)||AmpliSeq for Illumina Custom DNA Panel||AmpliSeq for Illumina On-Demand|
|Assay Time||~8 hours||As low as 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)||As low as 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)|
|Content Specifications||Design custom probes to sequence genomic regions of interest. Content range: 4 – 650 kb||Custom content from 1 (24 amplicons) to 500 (15,000 amplicons) genes|
|Hands-On Time||~4 hours||1.5 hrs|
|Input Quantity||50 – 250 ng high-quality genomic DNA; 250 ng FFPE DNA||1–100 ng (10 ng recommended per pool), 1–100 ng (10 ng recommended per pool)||1-100 ng DNA|
|Mechanism of Action||Probe hybridization, extension-ligation, and PCR||Multiplex PCR|
|Method||Amplicon Sequencing , Custom Sequencing||Amplicon Sequencing , Custom Sequencing, Targeted DNA Sequencing , Targeted RNA Sequencing||Amplicon Sequencing , Targeted DNA Sequencing|
|Multiplexing||1–96||Up to 96-plex|
|Specialized Sample Types||FFPE Tissue||Blood, FFPE Tissue||FFPE Tissue|
|Species Category||Bovine, Human, Mouse, Other, Rat||Any Species, Bovine, Canine, Chicken, Hamster, Human, Maize, Mouse, Ovine, Porcine, Rice, Soybean, Tomato||Human|
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The TruSeq Custom Amplicon assay is a simple and streamlined method for capturing and amplifying targeted regions of interest.
|Technology||TruSeq Custom Amplicon v1.5||TruSeq Custom Amplicon Low Input|
|Amplicon plexity (single pool)||Up to 10,000 (via Concierge)||Up to 1536|
|Input requirement||50 ng||as low as 10 ng|
|FFPE Compatibility||Yes – 250 ng||Yes – 10 ng (FFPE DNA input dependent on quality)|
|Amplicon size||150, 175, 250, 425 bp||150, 175, 250 bp|
|Instrument||MiniSeq, MiSeq, NextSeq||MiniSeq, MiSeq, NextSeq|
|Read Length||Up to 2 x 300 bp||Up to 2 x 150 bp|
|Time||10 hours||6.5 hours|
|Kit size (samples)||96+||16, 96+|
Two TruSeq Custom Amplicon options for targeted sequencing are available: TruSeq Custom Amplicon Low Input for low-input DNA and FFPE, or TruSeq Custom Amplicon 1.5 for very high plexity pools or longer amplicons. TruSeq Custom Amplicon Low Input enables you to detect and discover high-quality information, even from limited and challenging samples. Use the table to compare assays.